Papers

Peer-reviewed
Sep, 2000

Differential expression of mouse alpha 5(IV) and alpha 6(IV) collagen genes in epithelial basement membranes

JOURNAL OF BIOCHEMISTRY
  • K Saito
  • ,
  • Naito, I
  • ,
  • T Seki
  • ,
  • T Oohashi
  • ,
  • E Kimura
  • ,
  • R Momota
  • ,
  • Y Kishiro
  • ,
  • Y Sado
  • ,
  • H Yoshioka
  • ,
  • Y Ninomiya

Volume
128
Number
3
First page
427
Last page
434
Language
English
Publishing type
Research paper (scientific journal)
Publisher
JAPANESE BIOCHEMICAL SOC

We first completed the primary structure of the mouse alpha 5(IV) and alpha 6(IV) chains, from which synthetic peptides were produced and alpha chain-specific monoclonal antibodies were raised. Expression of collagen IV genes in various basement membranes underlying specific organ epithelia was analyzed by immunohistochemical staining using these monoclonal antibodies and other antibodies from human and bovine sequences. It was possible to predict the presence of the three collagen TV molecules: [alpha 1(IV)](2) alpha 2(IV), alpha 3(IV)alpha 4(IV)alpha 5(IV), and [alpha 5(IV)](2)alpha 6(IV), In skin basement membrane two of the three forms, [alpha 1(IV)](2)alpha 2(IV) and [alpha 5(IV)](2)alpha 6(IV), were detected. The alpha 3(IV)alpha 4(IV)alpha 5(IV) molecule was observed as the major form in glomerulus, alveolus, and choroid plexus, where basement membranes function as filtering units. The molecular form [alpha 5(IV)](2)alpha 6(IV) was present in basement membranes in tubular organs such as the epididymis, where the tubes need to expand in diameter. Thus, the distribution of the basement membranes with different molecular composition is consistent with tissue-specific function.


Link information
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000089147000012&DestApp=WOS_CPL
URL
http://orcid.org/0000-0002-7003-3444
ID information
  • ISSN : 0021-924X
  • ORCID - Put Code : 9101220
  • Web of Science ID : WOS:000089147000012

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