Copyright © 2018 by ISFA It is expected that the S-shape machining test which has proposed to ISO standard can be an effective method of accuracy evaluation for five-axis machining centers. However, there are few research works done for the dynamic error influence on the S-shape machining accuracy. This study investigates the influences of federate, control mode of NC controller and position loop gain of feed drive systems of a five-axis machining center. Actual S-shaped machining motions are carried out to this investigation. As the result, the effect caused by feed rate and machine control function is clarified. Combination of the control mode and position loop gain significantly influence the S-shape machining accuracy. It can be concluded that the proposed S-shape machining test could be used to evaluate the total performance including the control mode of the five-axis machining centers.