論文

査読有り
2020年7月30日

Roseobacter cerasinus sp. nov., isolated from a fish farm

International Journal of Systematic and Evolutionary Microbiology
  • So Muramatsu
  • ,
  • Masataka Kanamuro
  • ,
  • Yuki Sato-Takabe
  • ,
  • Setsuko Hirose
  • ,
  • Yuki Muramatsu
  • ,
  • Shinichi Takaichi
  • ,
  • Satoshi Hanada

記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1099/ijsem.0.004360
出版者・発行元
Microbiology Society

An obligate aerobic and bacteriochlorophyll <italic>a</italic>-containing bacterium, designated strain AI77T, was isolated from a fish farm in Uwa Sea, Japan. Cells were Gram-stain-negative, coccoid- to oval-shaped, and showed no motility. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain AI77T is a member of the genus <italic>
<named-content content-type="genus">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1134" xlink:type="simple">Roseobacter</ext-link>
</named-content>
</italic> and closely related to <italic>
<named-content content-type="species">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.30277" xlink:type="simple">Roseobacter ponti</ext-link>
</named-content>
</italic> MM-7T (97.8 %), <italic>
<named-content content-type="species">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1137" xlink:type="simple">Roseobacter denitrificans</ext-link>
</named-content>
</italic> OCh 114T (97.3 %) and <italic>
<named-content content-type="species">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1135" xlink:type="simple">Roseobacter litoralis</ext-link>
</named-content>
</italic> OCh 149T (97.3 %). The G+C content of strain AI77T was 61.0 mol%. The average amino acid identity values of the genome in strain AI77T with those in <italic>
<named-content content-type="species">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1137" xlink:type="simple">R. denitrificans</ext-link>
</named-content>
</italic> OCh 114T and <italic>
<named-content content-type="species">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1135" xlink:type="simple">R. litoralis</ext-link>
</named-content>
</italic> OCh 149T were 73.26 % (SD 16.46) and 72.63 % (SD 16.76), respectively. The digital DNA–DNA hybridization values of strain AI77T with the type strains <italic>
<named-content content-type="species">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1137" xlink:type="simple">R. denitrificans</ext-link>
</named-content>
</italic> OCh 114T and <italic>
<named-content content-type="species">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1135" xlink:type="simple">R. litoralis</ext-link>
</named-content>
</italic> OCh 149T were 18.70 and 18.50 %, respectively. The dominant fatty acids (&gt;10 % of total fatty acids) of AI77T were summed feature 8 (C18 : 1 ω7<italic>c</italic> and/or C18 : 1 ω6<italic>c</italic>) and saturated fatty acid C16 : 0. The sole respiratory quinone was ubiquinone-10. The predominant polar lipids were phosphatidylcholine, phosphatidylglycerol and diphosphatidylglycerol. Based on the genetic and phenotypic data obtained herein, we conclude that strain AI77T represents a new species of the genus <italic>
<named-content content-type="genus">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1134" xlink:type="simple">Roseobacter</ext-link>
</named-content>
</italic>, for which we propose the name <italic>Roseobacter cerasinus</italic> sp. nov.; the type strain is AI77T (=DSM 110091T=NBRC 114115T).

リンク情報
DOI
https://doi.org/10.1099/ijsem.0.004360
URL
https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004360?crawler=true

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