論文

査読有り 国際誌
2017年6月28日

Polyphenols from Lonicera caerulea L. Berry Inhibit LPS-Induced Inflammation through Dual Modulation of Inflammatory and Antioxidant Mediators.

Journal of agricultural and food chemistry
  • Shusong Wu
  • ,
  • Satoshi Yano
  • ,
  • Jihua Chen
  • ,
  • Ayami Hisanaga
  • ,
  • Kozue Sakao
  • ,
  • Xi He
  • ,
  • Jianhua He
  • ,
  • De-Xing Hou

65
25
開始ページ
5133
終了ページ
5141
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/acs.jafc.7b01599

Lonicera caerulea L. berry polyphenols (LCBP) are considered as major components for bioactivity. This study aimed to clarify the molecular mechanisms by monitoring inflammatory and antioxidant mediator actions in lipopolysaccharide (LPS)-induced mouse paw edema and macrophage cell model. LCBP significantly attenuated LPS-induced paw edema (3.0 ± 0.1 to 2.8 ± 0.1 mm, P < 0.05) and reduced (P < 0.05) serum levels of monocyte chemotactic protein-1 (MCP-1, 100.9 ± 2.3 to 58.3 ± 14.5 ng/mL), interleukin (IL)-10 (1596.1 ± 424.3 to 709.7 ± 65.7 pg/mL), macrophage inflammatory protein (MIP)-1α (1761.9 ± 208.3 to 1369.1 ± 56.4 pg/mL), IL-6 (1262.8 ± 71.7 to 499.0 ± 67.1 pg/mL), IL-4 (93.3 ± 25.7 to 50.7 ± 12.5 pg/mL), IL-12(p-70) (580.4 ± 132.0 to 315.2 ± 35.1 pg/mL), and tumor necrosis factor-α (TNF-α, 2045.5 ± 264.9 to 1270.7 ± 158.6 pg/mL). Cell signaling analysis revealed that LCBP inhibited transforming growth factor β activated kinase-1 (TAK1)-mediated mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) pathways, and enhanced the expression of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and manganese-dependent superoxide dismutase (MnSOD) in earlier response. Moreover, cyanidin 3-glucoside (C3G) and (-)-epicatechin (EC), two major components of LCBP, directly bound to TAK1. These data demonstrated that LCBP might inhibit LPS-induced inflammation by modulating both inflammatory and antioxidant mediators.

リンク情報
DOI
https://doi.org/10.1021/acs.jafc.7b01599
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28573848
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85021409850&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85021409850&origin=inward
ID情報
  • DOI : 10.1021/acs.jafc.7b01599
  • ISSN : 0021-8561
  • eISSN : 1520-5118
  • PubMed ID : 28573848
  • SCOPUS ID : 85021409850

エクスポート
BibTeX RIS