論文

査読有り
2016年7月

Dual mechanisms of rapid expression of anxiety-related behavior in pilocarpine-treated epileptic mice

EPILEPSY RESEARCH
  • Shintaro Otsuka
  • ,
  • Taro Ohkido
  • ,
  • Makoto Itakura
  • ,
  • Shigeru Watanabe
  • ,
  • Saori Yamamori
  • ,
  • Yuuki Iida
  • ,
  • Masanori Saito
  • ,
  • Hitoshi Miyaoka
  • ,
  • Masami Takahashi

123
開始ページ
55
終了ページ
67
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.eplepsyres.2016.04.007
出版者・発行元
ELSEVIER SCIENCE BV

A mouse model of epilepsy was generated by inducing status epilepticus (SE) for either 1.5 or 4.5 h with pilocarpine to study anxiety-related behaviors, changes in the electroencephalogram of the cerebral cortex and hippocampus, and expression of hippocampal proteins. The viability and rate of success of SE induction were high in C57BL/6N mice but not in C57BL/6J mice. C57BL/6N mice were immotile during the first 2 days after SE; however, by the third day, most mice were recovered and exhibited strong anxiety related behaviors in response to the light/dark preference test and open field test. There was a striking difference in the temporal appearance of anxiety-related behavior between the two SE durations: 1.5 h SE mice exhibited strong anxiety-related behavior 3 days after SE that gradually attenuated over the next few weeks, whereas 4.5 h SE mice exhibited strong anxiety-related behavior 3 days after SE that persisted even at nearly 1 year after SE. Mice receiving both SE durations exhibited generalized seizures (GS) after SE; however, there was a marked difference in the timing and duration of GS appearance. Mice in the 4.5 h SE group exhibited spontaneous GS from 4 days to at least 96 days after SE. In contrast, mice in the 1.5 h SE group exhibited GS only within the first several days after SE; however, epileptic spike clusters continuously appeared in the cerebral cortex and hippocampus for up to twelve days after SE. Among the hippocampal proteins tested, only brain derived-neurotrophic factor (BDNF) exhibited altered expression in parallel with anxiety-related behavior. These results showed the possibility that BDNF expression in the hippocampus might cause anxiety-related behavior in adulthood. (C) 2016 Elsevier B.V. All rights reserved.

Web of Science ® 被引用回数 : 13

リンク情報
DOI
https://doi.org/10.1016/j.eplepsyres.2016.04.007
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000376832100008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.eplepsyres.2016.04.007
  • ISSN : 0920-1211
  • eISSN : 1872-6844
  • Web of Science ID : WOS:000376832100008

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