論文

査読有り
2010年6月

Regulation of Microbe-Associated Molecular Pattern-Induced Hypersensitive Cell Death, Phytoalexin Production, and Defense Gene Expression by Calcineurin B-Like Protein-Interacting Protein Kinases, OsCIPK14/15, in Rice Cultured Cells

PLANT PHYSIOLOGY
  • Takamitsu Kurusu
  • ,
  • Jumpei Hamada
  • ,
  • Hiroshi Nokajima
  • ,
  • Youichiro Kitagawa
  • ,
  • Masahiro Kiyoduka
  • ,
  • Akira Takahashi
  • ,
  • Shigeru Hanamata
  • ,
  • Ryoko Ohno
  • ,
  • Teruyuki Hayashi
  • ,
  • Kazunori Okada
  • ,
  • Jinichiro Koga
  • ,
  • Hirohiko Hirochika
  • ,
  • Hisakazu Yamane
  • ,
  • Kazuyuki Kuchitsu

153
2
開始ページ
678
終了ページ
692
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1104/pp.109.151852
出版者・発行元
AMER SOC PLANT BIOLOGISTS

Although cytosolic free Ca2+ mobilization induced by microbe/pathogen-associated molecular patterns is postulated to play a pivotal role in innate immunity in plants, the molecular links between Ca2+ and downstream defense responses still remain largely unknown. Calcineurin B-like proteins (CBLs) act as Ca2+ sensors to activate specific protein kinases, CBL-interacting protein kinases (CIPKs). We here identified two CIPKs, OsCIPK14 and OsCIPK15, rapidly induced by microbe-associated molecular patterns, including chitooligosaccharides and xylanase (Trichoderma viride/ethylene-inducing xylanase [TvX/EIX]), in rice (Oryza sativa). Although they are located on different chromosomes, they have over 95% nucleotide sequence identity, including the surrounding genomic region, suggesting that they are duplicated genes. OsCIPK14/15 interacted with several OsCBLs through the FISL/NAF motif in yeast cells and showed the strongest interaction with OsCBL4. The recombinant OsCIPK14/15 proteins showed Mn2+-dependent protein kinase activity, which was enhanced both by deletion of their FISL/NAF motifs and by combination with OsCBL4. OsCIPK14/15-RNAi transgenic cell lines showed reduced sensitivity to TvX/EIX for the induction of a wide range of defense responses, including hypersensitive cell death, mitochondrial dysfunction, phytoalexin biosynthesis, and pathogenesis-related gene expression. On the other hand, TvX/EIX-induced cell death was enhanced in OsCIPK15-overexpressing lines. Our results suggest that OsCIPK14/15 play a crucial role in the microbe-associated molecular pattern-induced defense signaling pathway in rice cultured cells.

Web of Science ® 被引用回数 : 67

リンク情報
DOI
https://doi.org/10.1104/pp.109.151852
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000278340200031&DestApp=WOS_CPL

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