論文

査読有り
2015年5月

Autophagy Supports Biomass Production and Nitrogen Use Efficiency at the Vegetative Stage in Rice

PLANT PHYSIOLOGY
  • Shinya Wada
  • ,
  • Yasukazu Hayashida
  • ,
  • Masanori Izumi
  • ,
  • Takamitsu Kurusu
  • ,
  • Shigeru Hanamata
  • ,
  • Keiichi Kanno
  • ,
  • Soichi Kojima
  • ,
  • Tomoyuki Yamaya
  • ,
  • Kazuyuki Kuchitsu
  • ,
  • Amane Makino
  • ,
  • Hiroyuki Ishida

168
1
開始ページ
60
終了ページ
U721
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1104/pp.15.00242
出版者・発行元
AMER SOC PLANT BIOLOGISTS

Much of the nitrogen in leaves is distributed to chloroplasts, mainly in photosynthetic proteins. During leaf senescence, chloroplastic proteins, including Rubisco, are rapidly degraded, and the released nitrogen is remobilized and reused in newly developing tissues. Autophagy facilitates the degradation of intracellular components for nutrient recycling in all eukaryotes, and recent studies have revealed critical roles for autophagy in Rubisco degradation and nitrogen remobilization into seeds in Arabidopsis (Arabidopsis thaliana). Here, we examined the function of autophagy in vegetative growth and nitrogen usage in a cereal plant, rice (Oryza sativa). An autophagy-disrupted rice mutant, Osatg7-1, showed reduced biomass production and nitrogen use efficiency compared with the wild type. While Osatg7-1 showed early visible leaf senescence, the nitrogen concentration remained high in the senescent leaves. N-15 pulse chase analysis revealed suppression of nitrogen remobilization during leaf senescence in Osatg7-1. Accordingly, the reduction of nitrogen available for newly developing tissues in Osatg7-1 likely led its reduced leaf area and tillers. The limited leaf growth in Osatg7-1 decreased the photosynthetic capacity of the plant. Much of the nitrogen remaining in senescent leaves of Osatg7-1 was in soluble proteins, and the Rubisco concentration in senescing leaves of Osatg7-1 was about 2.5 times higher than in the wild type. Transmission electron micrographs showed a cytosolic fraction rich with organelles in senescent leaves of Osatg7-1. Our results suggest that autophagy contributes to efficient nitrogen remobilization at the whole-plant level by facilitating protein degradation for nitrogen recycling in senescent leaves.

Web of Science ® 被引用回数 : 64

リンク情報
DOI
https://doi.org/10.1104/pp.15.00242
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25786829
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000356967800006&DestApp=WOS_CPL

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