論文

査読有り 国際誌
2020年

Impact of Autophagy on Gene Expression and Tapetal Programmed Cell Death During Pollen Development in Rice.

Frontiers in plant science
  • Shigeru Hanamata
  • ,
  • Jumpei Sawada
  • ,
  • Seijiro Ono
  • ,
  • Kazunori Ogawa
  • ,
  • Togo Fukunaga
  • ,
  • Ken-Ichi Nonomura
  • ,
  • Seisuke Kimura
  • ,
  • Takamitsu Kurusu
  • ,
  • Kazuyuki Kuchitsu

11
開始ページ
172
終了ページ
172
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3389/fpls.2020.00172

Autophagy has recently been shown to be required for tapetal programmed cell death (PCD) and pollen maturation in rice. A transcriptional regulatory network is also known to play a key role in the progression of tapetal PCD. However, the relationship between the gene regulatory network and autophagy in rice anther development is mostly unknown. Here, we comprehensively analyzed the effect of autophagy disruption on gene expression profile during the tapetal PCD in rice anther development using high-throughput RNA sequencing. Expression of thousands of genes, including specific transcription factors and several proteases required for tapetal degradation, fluctuated synchronously at specific stages during tapetal PCD progression in the wild-type anthers, while this fluctuation showed significant delay in the autophagy-deficient mutant Osatg7-1. Moreover, gene ontology enrichment analysis in combination with self-organizing map clustering as well as pathway analysis revealed that the expression patterns of a variety of organelle-related genes as well as genes involved in carbohydrate/lipid metabolism were affected in the Osatg7-1 mutant during pollen maturation. These results suggest that autophagy is required for proper regulation of gene expression and quality control of organelles and timely progression of tapetal PCD during rice pollen development.

リンク情報
DOI
https://doi.org/10.3389/fpls.2020.00172
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32210988
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068715

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