論文

査読有り 国際誌
2020年12月1日

Cell Cycle-Dependence of Autophagic Activity and Inhibition of Autophagosome Formation at M Phase in Tobacco BY-2 Cells.

International journal of molecular sciences
  • Shigeru Hanamata
  • ,
  • Takamitsu Kurusu
  • ,
  • Kazuyuki Kuchitsu

担当区分
筆頭著者
21
23
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/ijms21239166

Autophagy is ubiquitous in eukaryotic cells and plays an essential role in stress adaptation and development by recycling nutrients and maintaining cellular homeostasis. However, the dynamics and regulatory mechanisms of autophagosome formation during the cell cycle in plant cells remain poorly elucidated. We here analyzed the number of autophagosomes during cell cycle progression in synchronized tobacco BY-2 cells expressing YFP-NtATG8a as a marker for the autophagosomes. Autophagosomes were abundant in the G2 and G1 phases of interphase, though they were much less abundant in the M and S phases. Autophagosomes drastically decreased during the G2/M transition, and the CDK inhibitor roscovitine inhibited the G2/M transition and the decrease in autophagosomes. Autophagosomes were rapidly increased by a proteasome inhibitor, MG-132. MG-132-induced autophagosome formation was also markedly lower in the M phases than during interphase. These results indicate that the activity of autophagosome formation is differently regulated at each cell cycle stage, which is strongly suppressed during mitosis.

リンク情報
DOI
https://doi.org/10.3390/ijms21239166
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33271936

エクスポート
BibTeX RIS