2019年1月25日
Mesenchymal-Epithelial Interactome Analysis Reveals Essential Factors Required for Fibroblast-Free Alveolosphere Formation.
iScience
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- 巻
- 11
- 号
- 開始ページ
- 318
- 終了ページ
- 333
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.isci.2018.12.022
Lung epithelial cells and fibroblasts are key cell populations in lung development. Fibroblasts support type 2 alveolar epithelial cells (AEC2) in the developing and mature lung. However, fibroblast-AEC2 interactions have not been clearly described. We addressed this in the present study by time course serial analysis of gene expression sequencing (SAGE-seq) of epithelial cells and fibroblasts of developing and mature murine lungs. We identified lung fibroblast-epithelial interactions that potentially regulate alveologenesis and are mediated by fibroblast-expressed ligands and epithelial cell surface receptors. In the epithelial-fibroblast co-culture alveolosphere formation assay, single intervention against fibroblast-expressed ligand or associated signaling cascades promoted or inhibited alveolosphere growth. Adding the ligand-associated molecules fibroblast growth factor 7 and Notch ligand and inhibitors of bone morphogenetic protein 4, transforming growth factor β, and glycogen synthase kinase-3β to the culture medium enabled fibroblast-free alveolosphere formation. The results revealed the essential factors regulating fibroblast-AEC2 interactions.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.isci.2018.12.022
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/30639966
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329323
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000456942200023&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.isci.2018.12.022
- ISSN : 2589-0042
- PubMed ID : 30639966
- PubMed Central 記事ID : PMC6329323
- Web of Science ID : WOS:000456942200023