2017年9月
Efficient near infrared fluorescence detection of elastase enzyme using peptide-bound unsymmetrical squaraine dye
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
- ,
- ,
- ,
- ,
- 巻
- 27
- 号
- 17
- 開始ページ
- 4024
- 終了ページ
- 4029
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bmcl.2017.07.057
- 出版者・発行元
- PERGAMON-ELSEVIER SCIENCE LTD
Extended wavelength analyte-responsive fluorescent probes are highly desired for the imaging applications owing to their deep tissue penetration, and minimum interference from autofluorescence by biomolecules. Near infra-red (NIR) sensitive and self-quenching fluorescent probe based on the dye-peptide conjugate (SQ I PC) was designed and synthesized by facile and efficient one-pot synthetic route for the detection of Elastase activity. In the phosphate buffer solution, there was an efficient quenching of fluorescence of SQ 1 PC (86%) assisted by pronounced dye-dye interaction due to H-aggregate formation. Efficient and fast recovery of this quenched fluorescence of SQ 1 PC (> 50% in 30 s) was observed on hydrolysis of this peptide-dye conjugate by elastase enzyme. Presently designed NIR sensitive self quenching substrate offers the potential application for the detection of diseases related to proteases by efficient and fast detection of their activities. (C) 2017 Elsevier Ltd. All rights reserved.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.bmcl.2017.07.057
- ISSN : 0960-894X
- eISSN : 1464-3405
- Web of Science ID : WOS:000410462500016