2009年4月
COUP-TFII acts downstream of Wnt/beta-catenin signal to silence PPAR gamma gene expression and repress adipogenesis
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- 巻
- 106
- 号
- 14
- 開始ページ
- 5819
- 終了ページ
- 5824
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1073/pnas.0901676106
- 出版者・発行元
- NATL ACAD SCIENCES
Wnt signaling through beta-catenin and TCF maintains preadipocytes in an un-differentiated proliferative state; however, the molecular pathway has not been completely defined. By integrating gene expression microarray, chromatin immunoprecipitation-chip, and cell-based experimental approaches, we show that Wnt/beta-catenin signaling activates the expression of COUP-TFII which recruits the SMRT corepressor complex to the first introns located downstream from the first exons of both PPAR gamma 1 and gamma 2 mRNAs. This maintains the local chromatin in a hypoacetylated state and represses PPAR gamma gene expression to inhibit adipogenesis. Our experiments define the COUP-TFII/SMRT complex as a previously unappreciated component of the linear pathway that directly links Wnt/beta-catenin signaling to repression of PPAR gamma gene expression and the inhibition of adipogenesis.
- リンク情報
-
- DOI
- https://doi.org/10.1073/pnas.0901676106
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/19307559
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2667001
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000264967500066&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1073/pnas.0901676106
- ISSN : 0027-8424
- PubMed ID : 19307559
- PubMed Central 記事ID : PMC2667001
- Web of Science ID : WOS:000264967500066