論文

査読有り
2009年10月

Contribution of the LIM Domain and Nebulin-Repeats to the Interaction of Lasp-2 with Actin Filaments and Focal Adhesions

PLOS ONE
  • Hiroyuki Nakagawa
  • ,
  • Hiroshi Suzuki
  • ,
  • Satoshi Machida
  • ,
  • Junko Suzuki
  • ,
  • Kazuyo Ohashi
  • ,
  • Mingyue Jin
  • ,
  • Shigeaki Miyamoto
  • ,
  • Asako G. Terasaki

4
10
開始ページ
e7530
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0007530
出版者・発行元
PUBLIC LIBRARY SCIENCE

Lasp-2 binds to actin filaments and concentrates in the actin bundles of filopodia and lamellipodia in neural cells and focal adhesions in fibroblastic cells. Lasp-2 has three structural regions: a LIM domain, a nebulin-repeat region, and an SH3 domain; however, the region(s) responsible for its interactions with actin filaments and focal adhesions are still unclear. In this study, we revealed that the N-terminal fragment from the LIM domain to the first nebulin-repeat module (LIM-n1) retained actin-binding activity and showed a similar subcellular localization to full-length lasp-2 in neural cells. The LIM domain fragment did not interact with actin filaments or localize to actin filament bundles. In contrast, LIM-n1 showed a clear subcellular localization to filopodial actin bundles. Although truncation of the LIM domain caused the loss of F-actin binding activity and the accumulation of filopodial actin bundles, these truncated fragments localized to focal adhesions. These results suggest that lasp-2 interactions with actin filaments are mediated through the cooperation of the LIM domain and the first nebulin-repeat module in vitro and in vivo. Actin filament binding activity may be a major contributor to the subcellular localization of lasp-2 to filopodia but is not crucial for lasp-2 recruitment to focal adhesions.

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0007530
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19851499
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000271022300003&DestApp=WOS_CPL
ID情報
  • DOI : 10.1371/journal.pone.0007530
  • ISSN : 1932-6203
  • PubMed ID : 19851499
  • Web of Science ID : WOS:000271022300003

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