論文

査読有り
2008年1月

Lasp-2 expression, localization, and ligand interactions: A new Z-disc scaffolding protein

CELL MOTILITY AND THE CYTOSKELETON
  • Anke Zieseniss
  • ,
  • Asako G. Terasaki
  • ,
  • Carol C. Gregorio

65
1
開始ページ
59
終了ページ
72
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/cm.20244
出版者・発行元
WILEY-LISS

The nebulin family of actin-binding proteins plays an important role in actin filament dynamics in a variety of cells including striated muscle. We report here the identification of a new striated muscle Z-disc associated protein: lasp-2 (LIM and SH3 domain protein-2). Lasp-2 is the most recently identified member of the nebulin family. To evaluate the role of lasp-2 in striated muscle, lasp-2 gene expression and localization were studied in chick and mouse tissue, as well as in primary cultures of chick cardiac and skeletal myocytes. Lasp-2 mRNA was detected as early as chick embryonic stage 25 and lasp-2 protein was associated with developing pre-myofibril structures, Z-discs of mature myofibrils, focal adhesions, and intercalated discs of cultured cardiomyocytes. Expression of GFP-tagged lasp-2 deletion constructs showed that the C-terminal region of lasp-2 is important for its localization in striated muscle cells. Lasp-2 organizes actin filaments into bundles and interacts directly with the Z-disc protein alpha-actinin. These results are consistent with a function of lasp-2 as a scaffolding and actin filament organizing protein within striated muscle Z-discs.

Web of Science ® 被引用回数 : 26

リンク情報
DOI
https://doi.org/10.1002/cm.20244
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/17968983
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000252139400005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/cm.20244
  • ISSN : 0886-1544
  • PubMed ID : 17968983
  • Web of Science ID : WOS:000252139400005

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