Kanako Kuwasako

Profile Information

Affiliation: Faculty of Pharmacy Department of Pharmaceutical Sciences, Musashino University

Degree: Ph.D.(Hiroshima University)

Other name(s) (e.g. nickname): 行木香奈子
J-GLOBAL ID: 201701009775415132
researchmap Member ID: B000270917

Research Interests

pre-mRNAスプライシング

Research Areas

Research History

Apr, 2014 - Present

Faculty of Pharmacy, Department of Pharmaceutical Sciences, Musashino University
Apr, 2013 - Present

Researcher
Apr, 2013 - Mar, 2014

Faculty of Pharmacy, Department of Pharmaceutical Sciences, Musashino University
Mar, 2000 - Mar, 2008

RIKEN

Education

Apr, 1997 - Mar, 1999

Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science
Apr, 1993 - Mar, 1997

Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science

Papers

Structural insights into recognition of SL4, the UUCG stem-loop, of human U1 snRNA by the ubiquitin-like domain, including the C-terminal tail in the SF3A1 subunit of U2 snRNP

Nobukazu Nameki, Shin-ichi Terawaki, Masayuki Takizawa, Madoka Kitamura, Yutaka Muto, Kanako Kuwasako

The Journal of Biochemistry, Apr 24, 2023 Peer-reviewedCorresponding author
Structural basis for the interaction between the first <scp>SURP</scp> domain of the <scp>SF3A1</scp> subunit in <scp>U2 snRNP</scp> and the human splicing factor <scp>SF1</scp>

Nobukazu Nameki, Masayuki Takizawa, Takayuki Suzuki, Shoko Tani, Naohiro Kobayashi, Taiichi Sakamoto, Yutaka Muto, Kanako Kuwasako

Protein Science, 31(10), Oct, 2022 Peer-reviewedCorresponding author
1H, 13C, and 15N resonance assignments and solution structures of the KH domain of human ribosome binding factor A, mtRbfA, involved in mitochondrial ribosome biogenesis

Kanako Kuwasako, Sakura Suzuki, Nobukazu Nameki, Masayuki Takizawa, Mari Takahashi, Kengo Tsuda, Takashi Nagata, Satoru Watanabe, Akiko Tanaka, Naohiro Kobayashi …

Biomolecular NMR Assignments, Jun 6, 2022 Peer-reviewedLead author
1H, 13C and 15N resonance assignments and solution structures of the two RRM domains of Matrin-3

Fahu He, Kanako Kuwasako, Masayuki Takizawa, Mari Takahashi, Kengo Tsuda, Takashi Nagata, Satoru Watanabe, Akiko Tanaka, Naohiro Kobayashi, Takanori Kigawa …

Biomolecular NMR Assignments, 16(1) 41-49, Apr, 2022 Peer-reviewedLead author
Elucidation of the aberrant 3′ splice site selection by cancer-associated mutations on the U2AF1

Hisashi Yoshida, Sam-Yong Park, Gyosuke Sakashita, Yuko Nariai, Kanako Kuwasako, Yutaka Muto, Takeshi Urano, Eiji Obayashi

Nature Communications, 11(1) 4744-4744, Dec, 2020 Peer-reviewed
1H, 13C and 15N resonance assignment of the YTH domain of YTHDC2

Fahu He, Ryuta Endo, Kanako Kuwasako, Mari Takahashi, Kengo Tsuda, Takashi Nagata, Satoru Watanabe, Akiko Tanaka, Naohiro Kobayashi, Takanori Kigawa …

Biomolecular NMR Assignments, 15(1) 1-7, Sep 15, 2020 Peer-reviewed
Solution structure of the first RNA recognition motif domain of human spliceosomal protein SF3b49 and its mode of interaction with a SF3b145 fragment

Kanako Kuwasako, Nobukazu Nameki, Kengo Tsuda, Mari Takahashi, Atsuko Sato, Naoya Tochio, Makoto Inoue, Takaho Terada, Takanori Kigawa, Naohiro Kobayashi …

PROTEIN SCIENCE, 26(2) 280-291, Feb, 2017 Peer-reviewedLead authorCorresponding author
A novel 3 ' splice site recognition by the two zinc fingers in the U2AF small subunit

Hisashi Yoshida, Sam-Yong Park, Takashi Oda, Taeko Akiyoshi, Mamoru Sato, Mikako Shirouzu, Kengo Tsuda, Kanako Kuwasako, Satoru Unzai, Yutaka Muto …

GENES & DEVELOPMENT, 29(15) 1649-1660, Aug, 2015 Peer-reviewed
Glycolytic flux controls D-serine synthesis through glyceraldehyde-3-phosphate dehydrogenase in astrocytes

Masataka Suzuki, Jumpei Sasabe, Yurika Miyoshi, Kanako Kuwasako, Yutaka Muto, Kenji Hamase, Masaaki Matsuoka, Nobuaki Imanishi, Sadakazu Aiso

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 112(17) E2217-E2224, Apr, 2015 Peer-reviewed
Controlling the Fluorescence of Benzofuran-Modified Uracil Residues in Oligonucleotides by Triple-Helix Formation

Takashi Kanamori, Hiroki Ohzeki, Yoshiaki Masaki, Akihiro Ohkubo, Mari Takahashi, Kengo Tsuda, Takuhiro Ito, Mikako Shirouzu, Kanako Kuwasako, Yutaka Muto …

CHEMBIOCHEM, 16(1) 167-176, Jan, 2015 Peer-reviewed
Novel RNA recognition motif domain in the cytoplasmic polyadenylation element binding protein 3

Kengo Tsuda, Kanako Kuwasako, Takashi Nagata, Mari Takahashi, Takanori Kigawa, Naohiro Kobayashi, Peter Guentert, Mikako Shirouzu, Shigeyuki Yokoyama, Yutaka Muto

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 82(10) 2879-2886, Oct, 2014 Peer-reviewedLead author
RBFOX and SUP-12 sandwich a G base to cooperatively regulate tissue-specific splicing

Kanako Kuwasako, Mari Takahashi, Satoru Unzai, Kengo Tsuda, Seiko Yoshikawa, Fahu He, Naohiro Kobayashi, Peter Guentert, Mikako Shirouzu, Takuhiro Ito …

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 21(9) 778-786, Sep, 2014 Peer-reviewedLead author
Structural insight into the interaction of ADP-ribose with the PARP WWE domains

Fahu He, Kengo Tsuda, Mari Takahashi, Kanako Kuwasako, Takaho Terada, Mikako Shirouzu, Satoru Watanabe, Takanori Kigawa, Naohiro Kobayashi, Peter Guentert …

FEBS LETTERS, 586(21) 3858-3864, Nov, 2012 Peer-reviewed
Solution structure of the splicing factor motif of the human Prp18 protein

Fahu He, Makoto Inoue, Takanori Kigawa, Mari Takahashi, Kanako Kuwasako, Kengo Tsuda, Naohiro Kobayashi, Takaho Terada, Mikako Shirouzu, Peter Guentert …

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 80(3) 968-974, Mar, 2012 Peer-reviewed
Structural basis for the dual RNA-recognition modes of human Tra2-beta RRM

Kengo Tsuda, Tatsuhiko Someya, Kanako Kuwasako, Mari Takahashi, Fahu He, Satoru Unzai, Makoto Inoue, Takushi Harada, Satoru Watanabe, Takaho Terada …

NUCLEIC ACIDS RESEARCH, 39(4) 1538-1553, Mar, 2011 Peer-reviewedLead author
Structural Insight into the Zinc Finger CW Domain as a Histone Modification Reader

Fahu He, Takashi Umehara, Kohei Saito, Takushi Harada, Satoru Watanabe, Takashi Yabuki, Takanori Kigawa, Mari Takahashi, Kanako Kuwasako, Kengo Tsuda …

STRUCTURE, 18(9) 1127-1139, Sep, 2010 Peer-reviewed
Structural basis for the sequence-specific RNA-recognition mechanism of human CUG-BP1 RRM3

Kengo Tsuda, Kanako Kuwasako, Mari Takahashi, Tatsuhiko Someya, Makoto Inoue, Takaho Terada, Naohiro Kobayashi, Mikako Shirouzu, Takanori Kigawa, Akiko Tanaka …

NUCLEIC ACIDS RESEARCH, 37(15) 5151-5166, Aug, 2009 Peer-reviewedLead author
AU-rich RNA-binding induces changes in the quaternary structure of AUH

Kazuki Kurimoto, Kanako Kuwasako, Alan M. Sandercock, Satoru Unzai, Carol V. Robinson, Yutaka Muto, Shigeyuki Yokoyama

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 75(2) 360-372, May, 2009 Peer-reviewedLead author
Solution structure of the second RNA recognition motif (RRM) domain of murine T cell intracellular antigen-1 (TIA-1) and its RNA recognition mode

Kanako Kuwasako, Mari Takahashi, Naoya Tochio, Chikage Abe, Kengo Tsuda, Makoto Inoue, Takaho Terada, Mikako Shirouzu, Naohiro Kobayashi, Takanori Kigawa …

BIOCHEMISTRY, 47(24) 6437-6450, Jun, 2008 Peer-reviewedLead author
Complex assembly mechanism and an RNA-binding mode of the human p14-SF3b 155 spliceosomal protein complex identified by NMR solution structure and functional analyses

Kanako Kuwasako, Naoshi Dohmae, Mio Inoue, Mikako Shirouzu, Seiichi Taguchi, Peter Guntert, Bertrand Seraphin, Yutaka Muto, Shigeyuki Yokoyama

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 71(4) 1617-1636, Jun, 2008 Peer-reviewedLead author

Misc.

組織特異的にスプライシング反応を制御するRBFOXファミリーRNA結合タンパク質およびSUP-12によるRNAの協働的な認識の機構

桑迫香奈子, 高橋真梨, 黒柳秀人, 武藤裕

ライフサイエンス新着論文レビュー, Sep, 2014 Invited
Characterization of RNA aptamers against SRP19 protein having sequences different from SRP RNA

Y. Haraguchi, K. Kuwasako, Y. Muto, Y. Bessho, M. Nishimoto, S. Yokoyama, A. Kanai, G. Kawai, T. Sakamoto

Nucleic Acids Symposium Series, 53(1) 265-266, Sep 1, 2009 Peer-reviewed

Presentations

スプライシングタンパク質U2AF1によるイントロン認識機構のX線結晶構造解析

吉田尚史, 朴三用, 成相裕子, 坂下暁介, 桑迫香奈子, 武藤裕, 浦野健, 尾林栄治

日本生化学会大会, Nov, 2021
がんに関わるスプライシング因子の構造生物学的研究

桑迫香奈子

第64回日本薬学会関東支部大会, Sep, 2020
哺乳類生殖細胞形成時の減数分裂を制御するYTHDC2のm6A結合ドメインの溶液構造

瀧澤将行, He F, 桑迫香奈子, 武藤裕

第64回日本薬学会関東支部大会, Sep, 2020
RNA recognition motifドメインによる協働的なRNA認識機構の解明

桑迫香奈子, 武藤裕

第64回日本薬学会関東支部大会, Sep, 2020
スプライシング因子の複合体に結合するaptamerの特性解析

佐藤謙太郎, 柳澤拓也, 瀧澤将行, 天野亮, 武藤裕, 桑迫香奈子, 坂本泰一

第41回日本分子生物学会年会, Nov, 2018
スプライシング因子の複合体に結合するaptamerの結合能の解析

佐藤謙太郎, 柳澤拓也, 瀧澤将行, 天野亮, 武藤裕, 桑迫香奈子, 坂本泰一

2018年度日本生化学会関東支部例会, Jun, 2018
立体構造解析に向けたスプライシング因子複合体に結合するRNA配列の取得

柳澤拓也, 桑迫香奈子, 天野亮, 瀧澤将行, 武藤裕, 坂本泰一

第40回日本分子生物学会年会, Dec, 2017
スプライシング因子の複合体に対する aptamer の取得と解析

柳澤拓也, 桑迫香奈子, 徳田正明, 天野亮, 武藤裕, 坂本泰一

生化学会関東支部例会, Jun, 2017
In vitro selection of RNA aptamers to a splicing factor.

Takuya Yanagisawa, Kanako Kuwasako, Ryo Amano, Yutaka Muto, Taiichi Sakamoto

21st Annual Meeting of the RNA Society, Jun, 2016
Solution structures of the two RNA recognition motif (RRM) domains of the human spliceosomal protein SF3b49.

Kanako Kuwasako, Kengo Tsuda, Mari Takahashi, Atsuko Sato, Naoya Tochio, Makoto Inoue, Takaho Terada, Takanori Kigawa, Naohiro Kobayashi, Mikako Shirouzu, Takuhiro Ito, Taiichi Sakamoto, Nobukazu Nameki, Kaori Wakamatsu, Peter Güntert, Seizo Takahashi, Shigeyuki Yokoyama, Yutaka Muto

21st Annual Meeting of the RNA Society, Jun, 2016
選択的スプライシング制御因子RBFOXとSUP-12による協働的なRNA認識の構造生物学的研究

桑迫香奈子, 高橋真梨, 雲財悟, 津田健吾, 吉川征子, 何発虎, 小林直弘, Peter Guntert, 白水美香子, 伊藤拓宏, 田仲昭子, 横山茂之, 萩原正敏, 黒柳秀人, 武藤裕

第36回日本分子生物学会年会, Nov, 2014
選択的スプライシング制御因子ASD-1とSUP-12による協働的なRNA認識の構造基盤

桑迫香奈子, 高橋真梨, 雲財悟, 津田健吾, 吉川征子, 何発虎, 小林直弘, Peter Guntert, 白水美香子, 伊藤拓宏, 田仲昭子, 横山茂之, 萩原正敏, 黒柳秀人, 武藤裕

第16回日本RNA学会, Jul, 2014
RNA recognition mode of the RNA recognition motif (RRM) of the human spliceosomal protein.

K. Kuwasako, A. Sato, K. Tsuda, M Takahashi, T, Ito, M Inoue, T. Terada, T, Kigawa, M. Shirouzu, N. Nameki, K, Wakamastu, S. Takahashi, S. Yokoyama, Y. Muto

第35回日本分子生物学会年会, Dec, 2013
Structural insight into the interaction of ADP-ribose with the PARP WWE domains.

He F, Tsuda K, Takahashi M, Kuwasako K, Terada T, Shirouzu M, Watanabe S, Kigawa T, Kobayashi N, Güntert P, Yokoyama S, †Muto Y

第35回日本分子生物学会年会, Dec, 2012
二つのCCHC-タイプのZinc Fingerドメインをもつタンパク質のRNAの認識機構

高橋真梨, 染谷龍彦, 坂本泰一, 平川真未, 桑迫香奈子, 津田健吾, 何発虎, 渡部暁, 原田拓志, 井上真, 寺田貴帆, 白水美香子, 木川隆則, 河合剛太, 横山茂之, 武藤裕

第34回日本分子生物学会年会, Dec, 2011
Structural basis of the characteristic interactions between Ubc9 and the SP-RING domain of a human SUMO E3 ligase.

†He F, Matsuda N, Kimura M, Inoue M, Watanabe S, Harada T, Kigawa T, Tsuda K, Kuwasako K, Takahashi M, Kobayashi N, Tanaka A, Güntert P, Yokoyama S, Muto Y

第34回日本分子生物学会年会, Dec, 2011
mRNA前駆体スプライシングに関わるタンパク質のRRMドメインによるRNAの認識

桑迫香奈子, 佐藤敦子, 井上真, 寺田貴帆, 白水美香子, 木川隆則, 高橋征三, 横山茂之, 武藤裕

第34回日本分子生物学会年会, Dec, 2011
Structural Basis for the Dual RNA-recognition Modes of human Tra2-beta RRM.

Tsuda K, Someya T, Kuwasako K, Takahashi M, He F, Unzai S, Inoue M, Harada T, Watanabe S, Terada T, Kobayashi N, Shirouzu M, Kigawa T, Tanaka A, Sugano S, Guntert P, Yokoyama S, †Muto Y

The 16th Annual Meeting of the RNA Society, Jun, 2011
構造を基盤とした選択的スプライシング因子SUP-12のRNA認識の解明

高橋真梨, 桑迫香奈子, 津田健吾, 黒柳秀人, 萩原正敏, 横山茂之, 武藤裕

第33回日本分子生物学会年会・第83回日本生化学会大会合同大会, Dec, 2010
Solution Structure of Splicing Factor Motif (SFM) in Prp18 from Homo sapiens.

†He F, Inoue M, Kigawa T, Takahashi M, Kuwasako K, Tsuda K, Kobayashi N, Terada T, Shirouzu M, Tanaka A, Sugano S, Güntert P, Yokoyama S, Muto Y

第33回日本分子生物学会年会・第83回日本生化学会大会合同大会, Dec, 2010

Teaching Experience

Professional Memberships

Research Projects

RNAアプタマーを利用したU2 snRNPの「ゆるい」ブランチ部位認識機構の解明

科学研究費補助金基盤（C）, 日本学術振興会, 2022
脊髄性筋萎縮症に関わるスプライシング制御因子の協同作用の解明

科学研究費補助金基盤（C）, 日本学術振興会, 2021
スプライシング因子とブランチ部位の「ゆるい」相互作用メカニズムの解明

研究奨励（化学・生命分野）, 公益財団法人旭硝子財団, 2020
がん抑制に関わる選択的スプライシング制御蛋白質の標的遺伝子の同定と認識機構の解明

科学研究費補助金基盤（C）, 日本学術振興会, 2018
肺がん抑制に関わる選択的スプライシング制御タンパク質の標的RNA認識メカニズムの解明

内藤記念科学奨励金・研究助成, 公益財団法人内藤記念科学振興財団, Dec, 2017
がん細胞におけるSF3b1を介したスプライシング異常の研究

基礎科学研究助成, 基礎科学研究助成, 公益財団法人住友財団, Nov, 2015 - Nov, 2017
がん発症に関わるスプライシング因子SF3b1の構造生物学的研究

科学研究費補助金基盤（C）, 日本学術振興会, Apr, 2015 - Mar, 2017
がん細胞におけるスプライシング因子SF3b1の変異によるmRNA認識能の変化メカニズムの解明

薬学系研究奨励, 薬学系研究奨励, 武田科学振興財団, Jul, 2016

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