論文

国際誌
2021年5月11日

Low nitrogen conditions accelerate flowering by modulating the phosphorylation state of FLOWERING BHLH 4 in Arabidopsis.

Proceedings of the National Academy of Sciences of the United States of America
  • Miho Sanagi
  • ,
  • Shoki Aoyama
  • ,
  • Akio Kubo
  • ,
  • Yu Lu
  • ,
  • Yasutake Sato
  • ,
  • Shogo Ito
  • ,
  • Mitsutomo Abe
  • ,
  • Nobutaka Mitsuda
  • ,
  • Masaru Ohme-Takagi
  • ,
  • Takatoshi Kiba
  • ,
  • Hirofumi Nakagami
  • ,
  • Filip Rolland
  • ,
  • Junji Yamaguchi
  • ,
  • Takato Imaizumi
  • ,
  • Takeo Sato

118
19
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1073/pnas.2022942118

Nitrogen (N) is an essential nutrient that affects multiple plant developmental processes, including flowering. As flowering requires resources to develop sink tissues for reproduction, nutrient availability is tightly linked to this process. Low N levels accelerate floral transition; however, the molecular mechanisms underlying this response are not well understood. Here, we identify the FLOWERING BHLH 4 (FBH4) transcription factor as a key regulator of N-responsive flowering in Arabidopsis Low N-induced early flowering is compromised in fbh quadruple mutants. We found that FBH4 is a highly phosphorylated protein and that FBH4 phosphorylation levels decrease under low N conditions. In addition, decreased phosphorylation promotes FBH4 nuclear localization and transcriptional activation of the direct target CONSTANS (CO) and downstream florigen FLOWERING LOCUS T (FT) genes. Moreover, we demonstrate that the evolutionarily conserved cellular fuel sensor SNF1-RELATED KINASE 1 (SnRK1), whose kinase activity is down-regulated under low N conditions, directly phosphorylates FBH4. SnRK1 negatively regulates CO and FT transcript levels under high N conditions. Together, these results reveal a mechanism by which N levels may fine-tune FBH4 nuclear localization by adjusting the phosphorylation state to modulate flowering time. In addition to its role in flowering regulation, we also showed that FBH4 was involved in low N-induced up-regulation of nutrient recycling and remobilization-related gene expression. Thus, our findings provide insight into N-responsive growth phase transitions and optimization of plant fitness under nutrient-limited conditions.

リンク情報
DOI
https://doi.org/10.1073/pnas.2022942118
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33963081
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126780
ID情報
  • DOI : 10.1073/pnas.2022942118
  • PubMed ID : 33963081
  • PubMed Central 記事ID : PMC8126780

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