論文

国際誌
2020年

Protein Phosphorylation Dynamics Under Carbon/Nitrogen-Nutrient Stress and Identification of a Cell Death-Related Receptor-Like Kinase in Arabidopsis.

Frontiers in plant science
  • Xingwen Li
  • ,
  • Miho Sanagi
  • ,
  • Yu Lu
  • ,
  • Yuko Nomura
  • ,
  • Sara Christina Stolze
  • ,
  • Shigetaka Yasuda
  • ,
  • Yusuke Saijo
  • ,
  • Waltraud X Schulze
  • ,
  • Regina Feil
  • ,
  • Mark Stitt
  • ,
  • John E Lunn
  • ,
  • Hirofumi Nakagami
  • ,
  • Takeo Sato
  • ,
  • Junji Yamaguchi

11
開始ページ
377
終了ページ
377
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3389/fpls.2020.00377

Nutrient availability, in particular the availability of sugar [carbon (C)] and nitrogen (N), is important for the regulation of plant metabolism and development. In addition to independent utilization of C and N nutrients, plants sense and respond to the balance of C and N nutrients (C/N-nutrient) available to them. High C/low N-nutrient stress has been shown to arrest early post-germinative growth while promoting progression to senescence in Arabidopsis. Although several signaling components of the C/N-nutrient response have been identified, the inclusive molecular basis of plant C/N-nutrient response remains unclear. This proteome analysis evaluated phosphorylation dynamics in response to high C/low N-nutrient stress. Phosphoproteomics under conditions of C/N-nutrient stress showed a global change in the phosphorylation status of proteins, including plasma membrane H+-ATPase, carbon and nitrogen metabolic enzymes and signaling proteins such as protein kinases and transcription factors. Further analyses suggested that SNF1-related protein kinase 1 (SnRK1) is involved in primary C/N-nutrient signal mediation via the transcriptional regulation of C/N-regulatory kinases. We also identified a leucine-rich repeat receptor-like kinase with extracellular malectin-like domain, named as LMK1, which was shown to possess cell death induction activity in plant leaves. These results provide important insight into the C/N-nutrient signaling pathways connecting nutrition stress to various cellular and physiological processes in plants.

リンク情報
DOI
https://doi.org/10.3389/fpls.2020.00377
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32308664
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7145971
ID情報
  • DOI : 10.3389/fpls.2020.00377
  • PubMed ID : 32308664
  • PubMed Central 記事ID : PMC7145971

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