Research Projects

Apr, 2017 - Mar, 2020

Development of cell-permeable oligonucleotides bearing bio-cleavable protecting groups

Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

Grant number
Japan Grant Number (JGN)
Grant amount
4,680,000 Japanese Yen
(Direct funding)
3,600,000 Japanese Yen
(Indirect funding)
1,080,000 Japanese Yen

Objective of this project is to develop synthetic oligonucleotides with high cell-membrane permeability and activatable property. In research period, glutathione-labile protecting groups for phosphodiester moiety in oligonucleotides were developed. Oligonucleotides modified with glutathione-labile protecting groups showed cell-membrane permeability without use of transfection reagent. The cell-membrane permeability increased depending on the number of protecting groups. Moreover the location of protecting groups was also influenced on the cellular uptake. Internalized oligonucleoides were activated and transformed into biologically active oligonucleotides.

Link information
ID information
  • Grant number : 17K01966
  • Japan Grant Number (JGN) : JP17K01966