論文

査読有り 国際誌
2018年9月

Gastric gland mucin-specific O-glycan expression decreases as tumor cells progress from lobular endocervical gland hyperplasia to cervical mucinous carcinoma, gastric type

VIRCHOWS ARCHIV
  • Kazuhiro Yamanoi
  • ,
  • Keiko Ishii
  • ,
  • Michihiko Tsukamoto
  • ,
  • Shiho Asaka
  • ,
  • Jun Nakayama

473
3
開始ページ
305
終了ページ
311
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00428-018-2381-6
出版者・発行元
SPRINGER

Gastric gland mucin-specific O-glycans are unique in having alpha 1,4-linked N-acetylglucosamine (alpha GlcNAc) attached to MUC6. We previously reported decreased expression of alpha GlcNAc relative to MUC6 in gastric and pancreatic neoplasms, but its significance in cervical glandular lesions remained unclear. Here, we analyzed MUC5AC, MUC6, alpha GlcNAc, and p16 expression in 9 lesions of mucinous carcinoma, gastric type with minimal deviation adenocarcinoma (GAS-MDA), 5 of GAS with nonMDA (GAS-nonMDA), 14 of typical lobular endocervical gland hyperplasia (LEGH), and 5 of atypical LEGH (33 total lesions). All 33 were MUC5AC-positive. Moreover, all 14 typical LEGH, 5 atypical LEGH, 8 of 9 GAS-MDA, and 3 of 5 GAS-nonMDA were MUC6-positive. All 14 typical LEGH, 2 of 5 atypical LEGH, 3 of 9 GAS-MDA, and 1 of 5 GAS-nonMDA were alpha GlcNAc-positive. The proportion of alpha GlcNAc-positive atypical LEGH or GAS-MDA or GAS-nonMDA lesions was significantly smaller than that seen in typical LEGH lesions (P < 0.001 and P < 0.01, respectively). Of 33 lesions, 32 were p16-negative. Furthermore, when we evaluated MUC6 and alpha GlcNAc immunoreactivity semi-quantitatively in all 33 lesions, in typical LEGH and GAS-MDA, the immunohistochemical score for alpha GlcNAc was significantly lower than that for MUC6 (P < 0.01). We did not observe significantly decreased alpha GlcNAc expression relative to MUC6 in typical LEGH lesions. These studies suggest that alpha GlcNAc expression decreases as typical LEGH progresses to GAS. Given the difficulty in distinguishing MDA and atypical LEGH from typical LEGH in H.E. staining, we propose that immunohistochemical analysis of alpha GlcNAc and MUC6 could be useful.

Web of Science ® 被引用回数 : 10

リンク情報
DOI
https://doi.org/10.1007/s00428-018-2381-6
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29845361
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000443403100005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00428-018-2381-6
  • ISSN : 0945-6317
  • eISSN : 1432-2307
  • ORCIDのPut Code : 50664871
  • PubMed ID : 29845361
  • Web of Science ID : WOS:000443403100005

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