論文

査読有り 筆頭著者 国際誌
2018年2月

Utility of bronchial lavage fluids for epithelial growth factor receptor mutation assay in lung cancer patients: Comparison between cell pellets, cell blocks and matching tissue specimens.

Oncology letters
  • Shiho Asaka
  • ,
  • Akihiko Yoshizawa
  • ,
  • Rie Nakata
  • ,
  • Tatsuya Negishi
  • ,
  • Hiroshi Yamamoto
  • ,
  • Takayuki Shiina
  • ,
  • Shohei Shigeto
  • ,
  • Kazuyuki Matsuda
  • ,
  • Yukihiro Kobayashi
  • ,
  • Takayuki Honda

15
2
開始ページ
1469
終了ページ
1474
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3892/ol.2017.7464

The detection of epidermal growth factor receptor (EGFR) mutations is necessary for the selection of suitable patients with non-small cell lung cancer (NSCLC) for treatment with EGFR tyrosine kinase inhibitors. Cytology specimens are known to be suitable for EGFR mutation detection, although tissue specimens should be prioritized; however, there are limited studies that examine the utility of bronchial lavage fluid (BLF) in mutation detection. The purpose of the present study was to investigate the utility of BLF specimens for the detection of EGFR mutations using a conventional quantitative EGFR polymerase chain reaction (PCR) assay. Initially, quantification cycle (Cq) values of cell pellets, cell-free supernatants and cell blocks obtained from three series of 1% EGFR mutation-positive lung cancer cell line samples were compared for mutation detection. In addition, PCR analysis of BLF specimens obtained from 77 consecutive NSCLC patients, detecting EGFR mutations was validated, and these results were compared with those for the corresponding formalin-fixed paraffin-embedded (FFPE) tissue specimens obtained by surgical resection or biopsy of 49 of these patients. The Cq values for mutation detection were significantly lower in the cell pellet group (average, 29.58) compared with the other groups, followed by those in cell-free supernatants (average, 34.15) and in cell blocks (average, 37.12) for all three series (P<0.05). Mutational status was successfully analyzed in 77 BLF specimens, and the results obtained were concordant with those of the 49 matching FFPE tissue specimens. Notably, EGFR mutations were even detected in 10 cytological specimens that contained insufficient tumor cells. EGFR mutation testing with BLF specimens is therefore a useful and reliable method, particularly when sufficient cancer cells are not obtained.

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.3892/ol.2017.7464
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29399190
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5774526
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000426145000013&DestApp=WOS_CPL
ID情報
  • DOI : 10.3892/ol.2017.7464
  • ORCIDのPut Code : 50664271
  • PubMed ID : 29399190
  • PubMed Central 記事ID : PMC5774526
  • Web of Science ID : WOS:000426145000013

エクスポート
BibTeX RIS