論文

査読有り
2018年

Isolation, Characterization and PCR Multiplexing of Microsatellite Loci for Western Sand Lance (Ammodytes japonicus Duncker and Mohr 1939)

JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY
  • Jun-ya Shibata
  • ,
  • Satoshi Tomano
  • ,
  • Tetsuya Umino
  • ,
  • Takeshi Tomiyama
  • ,
  • Yoichi Sakai
  • ,
  • Satoshi Nakai
  • ,
  • Tetsuji Okuda
  • ,
  • Wataru Nishijima

52
4
開始ページ
307
終了ページ
313
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.6090/jarq.52.307
出版者・発行元
JAPAN INT RESEARCH CENTER AGRICULTURAL SCIENCES

Ten novel microsatellite markers were developed for the western sand lance, Ammodytes japonicus, which has decreased significantly in many fishing grounds because of overexploitation and deterioration of its habitat. The 10 markers were successfully amplified in two sets of multiplex polymerase chain reactions (PCR). A total of 63 individuals collected in two successive years from a single population were used to assess the characteristics of the 10 markers. The number of alleles per locus ranged from seven to 24 with the observed heterozygosity ranging from 0.397 to 0.921. None of the loci deviated significantly from the Hardy-Weinberg equilibrium, and there was no evidence of linkage disequilibrium between any loci-pairs. Almost all of these novel microsatellite markers were also confirmed to be successfully amplified, not only for the other regional A. japonicus, but also for the closely-related Ammodytes heian and Ammodytes hexapterus. These polymorphic microsatellite markers for multiplex PCR will largely improve the throughput of microsatellite DNA analysis, and contribute to the effective genetic monitoring of A. japonicus and other sand lances around Japan.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.6090/jarq.52.307
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000457117700004&DestApp=WOS_CPL

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