論文

査読有り 筆頭著者
2018年8月20日

RGB-Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells

Angewandte Chemie International Edition
  • Satoshi Arai
  • Rókus Kriszt
  • Kazuki Harada
  • Liang Sheng Looi
  • Shogo Matsuda
  • Devina Wongso
  • Satoshi Suo
  • Shoichi Ishiura
  • Yu Hua Tseng
  • Michael Raghunath
  • Toshiro Ito
  • Takashi Tsuboi
  • Tetsuya Kitaguchi
  • 全て表示

57
34
開始ページ
10873
終了ページ
10878
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/anie.201804304

© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Adenosine triphosphate (ATP) provides energy for the regulation of multiple cellular processes in living organisms. Capturing the spatiotemporal dynamics of ATP in single cells is fundamental to our understanding of the mechanisms underlying cellular energy metabolism. However, it has remained challenging to visualize the dynamics of ATP in and between distinct intracellular organelles and its interplay with other signaling molecules. Using single fluorescent proteins, multicolor ATP indicators were developed, enabling the simultaneous visualization of subcellular ATP dynamics in the cytoplasm and mitochondria of cells derived from mammals, plants, and worms. Furthermore, in combination with additional fluorescent indicators, the dynamic interplay of ATP, cAMP, and Ca2+ could be visualized in activated brown adipocyte. This set of indicator tools will facilitate future research into energy metabolism.

リンク情報
DOI
https://doi.org/10.1002/anie.201804304
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29952110
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85050698563&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85050698563&origin=inward
ID情報
  • DOI : 10.1002/anie.201804304
  • ISSN : 1433-7851
  • eISSN : 1521-3773
  • PubMed ID : 29952110
  • SCOPUS ID : 85050698563

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