論文

査読有り 国際誌
2016年11月

Distribution of type VI collagen in association with osteoblast lineages in the groove of Ranvier during rat postnatal development.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft
  • Yukihiro Kohara
  • ,
  • Satoshi Soeta
  • ,
  • Yayoi Izu
  • ,
  • Kiyotaka Arai
  • ,
  • Hajime Amasaki

208
開始ページ
58
終了ページ
68
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.aanat.2016.07.003

In the groove of Ranvier (GOR), osteoblast lineages form bone bark, which develops into endosteal cortical bone. This ossification process is thought to be regulated by the microenvironment in the GOR. Type VI collagen (Col VI), an extracellular matrix (ECM) protein found in the periosteum/perichondrium, mediates osteoblast differentiation via the cell-surface receptor neural/glial antigen 2 (NG2) chondroitin sulfate proteoglycan. In order to clarify the function of Col VI during osteoblast differentiation in the GOR, in the present study, we examined the distribution of Col VI and osteoblast lineages expressing NG2 in the rat tibia proximal end during postnatal growing periods by immunohistochemistry. Our data revealed that Col VI accumulated in the ECM of the GOR middle layer and that Col VI accumulation was reduced and disappeared in the inner and middle lower regions. Runt-related transcription factor 2-immunoreactive pre-osteoblasts expressed NG2 in Col VI-immunopositive areas. However, Osterix-immunoreactive mature osteoblasts were only found in the Col VI-immunonegative area. These findings indicate that Col VI provided a characteristic microenvironment in the GOR and that NG2-Col VI interactions may regulate the differentiation of osteoblast lineages prior to terminal maturation.

リンク情報
DOI
https://doi.org/10.1016/j.aanat.2016.07.003
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27498042
ID情報
  • DOI : 10.1016/j.aanat.2016.07.003
  • ISSN : 0940-9602
  • PubMed ID : 27498042

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