論文

査読有り
2014年12月1日

Molecular analysis of the koala reproductive hormones and their receptors: Gonadotrophin-releasing hormone (GnRH), follicle-stimulating hormone β and luteinising hormone β with localisation of GnRH

Journal of Neuroendocrinology
  • E. R. Busby
  • ,
  • S. Soeta
  • ,
  • N. M. Sherwood
  • ,
  • S. D. Johnston

26
12
開始ページ
870
終了ページ
887
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/jne.12208
出版者・発行元
Blackwell Publishing Ltd

During evolution, reproductive hormones and their receptors in the brain-pituitary-gonadal axis have been altered by genetic mechanisms. To understand how the neuroendocrine control of reproduction evolved in mammals, it is important to examine marsupials, the closest group to placental mammals. We hypothesised that at least some of the hormones and receptors found in placental mammals would be present in koala, a marsupial. We examined the expression of koala mRNA for the reproductive molecules. Koala cDNAs were cloned from brain for gonadotrophin-releasing hormones (GnRH1 and GnRH2) or from pituitary for GnRH receptors, types I and II, follicle-stimulating hormone (FSH)β and luteinising hormone (LH)β, and from gonads for FSH and LH receptors. Deduced proteins were compared by sequence alignment and phylogenetic analysis with those of other vertebrates. In conclusion, the koala expressed mRNA for these eight putative reproductive molecules, whereas at least one of these molecules is missing in some species in the amniote lineage, including humans. In addition, GnRH1 and 2 are shown by immunohistochemistry to be expressed as proteins in the brain.

リンク情報
DOI
https://doi.org/10.1111/jne.12208
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25200132
ID情報
  • DOI : 10.1111/jne.12208
  • ISSN : 1365-2826
  • ISSN : 0953-8194
  • PubMed ID : 25200132
  • SCOPUS ID : 84910602725

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