2005年9月
Distinct functional units of the Golgi complex in Drosophila cells
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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- 巻
- 102
- 号
- 38
- 開始ページ
- 13467
- 終了ページ
- 13472
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1073/pnas.0506681102
- 出版者・発行元
- NATL ACAD SCIENCES
A striking variety of glycosylation occur in the Golgi complex in a protein-specific manner, but how this diversity and specificity are achieved remains unclear. Here we show that stacked fragments (units) of the Golgi complex dispersed in Drosophila imaginal disk cells are functionally diverse. The UDP-sugar transporter FRINGE-CONNECTION (FRC) is localized to a subset of the Golgi units distinct from those harboring SULFATELESS (SFL), which modifies glucosaminoglycans (GAGs), and from those harboring the protease RHOMBOID (RHO), which processes the glycoprotein SPITZ (SPI). Whereas the glycosylation and function of NOTCH are affected in imaginal disks of frc mutants, those of SPI and of GAG core proteins are not, even though FRC transports a broad range of glycosylation substrates, suggesting that Golgi units containing FRC and those containing SFL or RHO are functionally separable. Distinct Golgi units containing FRC and RHO in embryos could also be separated biochemically by immunoisolation techniques. We also show that Tn-antigen glycan is localized only in a subset of the Golgi units distributed basally in a polarized cell. We propose that the different localizations among distinct Golgi units of molecules involved in glycosylation underlie the diversity of glycan modification.
- リンク情報
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- DOI
- https://doi.org/10.1073/pnas.0506681102
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/16174741
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1224666
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000232115100022&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1073/pnas.0506681102
- ISSN : 0027-8424
- PubMed ID : 16174741
- PubMed Central 記事ID : PMC1224666
- Web of Science ID : WOS:000232115100022