2007年4月
Taurine transporter from the giant Pacific oyster Crassostrea gigas: function and expression in response to hyper- and hypo-osmotic stress
FISHERIES SCIENCE
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- 巻
- 73
- 号
- 2
- 開始ページ
- 385
- 終了ページ
- 394
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/j.1444-2906.2007.01346.x
- 出版者・発行元
- BLACKWELL PUBLISHING
Taurine is the primary osmolyte in marine molluscs, whose cellular osmo-conforming process is vital for environmental adaptation because of a lack of osmotic homeostasis. Here, cDNA cloning and expression, and functional analyses of taurine transporter (TAUT) from the giant Pacific oyster are reported on. The deduced amino-acid sequence of oyster TAUT (oyTAUT) showed 47-51% identity to those of vertebrate TAUT, whereas identity among the vertebrates is 78-95%. Functional analysis of oyTAUT expressed in Xenopus oocytes revealed that oyTAUT has a lower affinity and specificity for taurine and a requirement for higher NaCl concentration, compared with vertebrate TAUT. Taken together with similar functional properties of TAUT from mussel, indicated by our previous study, it is possible that these functional features reflect the internal environment of the molluscs (i.e. higher taurine and NaCl concentrations). Oyster taurine transporter mRNA expression was induced by not only hyper-osmotic stress, similar to other TAUT, but also hypo-osmotic stress. It is speculated that the expression in response to hypo-osmotic stress was induced by a substantial decrease in tissue taurine content following the decrease in the internal osmolality.
- リンク情報
- ID情報
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- DOI : 10.1111/j.1444-2906.2007.01346.x
- ISSN : 0919-9268
- CiNii Articles ID : 10018417509
- Web of Science ID : WOS:000246016600020