論文

査読有り 国際誌
2019年4月8日

Soritesidine, a Novel Proteinous Toxin from the Okinawan Marine Sponge Spongosorites sp.

Marine drugs
  • Ryuichi Sakai
  • ,
  • Kota Tanano
  • ,
  • Takumi Ono
  • ,
  • Masaya Kitano
  • ,
  • Yusuke Iida
  • ,
  • Koji Nakano
  • ,
  • Mitsuru Jimbo

17
4
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/md17040216

A novel protein, soritesidine (SOR) with potent toxicity was isolated from the marine sponge Spongosorites sp. SOR exhibited wide range of toxicities over various organisms and cells including brine shrimp (Artemia salina) larvae, sea hare (Aplysia kurodai) eggs, mice, and cultured mammalian cells. Toxicities of SOR were extraordinary potent. It killed mice at 5 ng/mouse after intracerebroventricular (i.c.v.) injection, and brine shrimp and at 0.34 µg/mL. Cytotoxicity for cultured mammalian cancer cell lines against HeLa and L1210 cells were determined to be 0.062 and 12.11 ng/mL, respectively. The SOR-containing fraction cleaved plasmid DNA in a metal ion dependent manner showing genotoxicity of SOR. Purified SOR exhibited molecular weight of 108.7 kDa in MALDI-TOF MS data and isoelectric point of approximately 4.5. N-terminal amino acid sequence up to the 25th residue was determined by Edman degradation. Internal amino acid sequences for fifteen peptides isolated from the enzyme digest of SOR were also determined. None of those amino acid sequences showed similarity to existing proteins, suggesting that SOR is a new proteinous toxin.

リンク情報
DOI
https://doi.org/10.3390/md17040216
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30965587
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520796
ID情報
  • DOI : 10.3390/md17040216
  • PubMed ID : 30965587
  • PubMed Central 記事ID : PMC6520796

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