2014年
Recovery Correction Technique for NMR Spectroscopy of Perchloric Acid Extracts using DL-valine-2,3-d(2): Validation and Application to 5-Fluorouracil-induced Brain Damage
MAGNETIC RESONANCE IN MEDICAL SCIENCES
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- 巻
- 13
- 号
- 3
- 開始ページ
- 145
- 終了ページ
- 153
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.2463/mrms.2013-0089
- 出版者・発行元
- JPN SOC MAGNETIC RESONANCE MEDICINE
Purpose: We explored a recovery correction technique that can correct metabolite loss during perchloric acid (PCA) extraction and minimize inter-assay variance in quantitative H-1 nuclear magnetic resonance (NMR) spectroscopy of the brain and evaluated its efficacy in 5-fluorouracil (5-FU)- and saline-administered rats.Methods: We measured the recovery of creatine and DL-valine-2,3-d(2) from PCA extract containing both compounds (0.5 to 8 mM). We intravenously administered either 5-FU for 4 days (total, 100 mg/kg body weight) or saline into 2 groups of 11 rats each. We subsequently performed PCA extraction of the whole brain on Day 9, externally adding 7 mu mol of DL-valine-2,3-d(2). We estimated metabolite concentrations using an NMR spectrometer with recovery correction, correcting metabolite concentrations based on the recovery factor of DL-valine-2,3-d(2). For each metabolite concentration, we calculated the coefficient of variation (CEV) and compared differences between the 2 groups using unpaired t-test.Results: Equivalent recoveries of DL-valine-2,3-d(2) (89.4 +/- 3.9%) and creatine (89.7 +/- 3.9%) in the PCA extract of the mixed solution indicated the suitability of DL-valine-2,3-d(2) as an internal reference. In the rat study, recovery of DL-valine-2,3-d(2) was 90.6 +/- 9.2%. Nine major metabolite concentrations adjusted by recovery of DL-valine-2,3-d(2) in saline-administered rats were comparable to data in the literature. CEVs of these metabolites were reduced from 10 to 17% before to 7 to 16% after correction. The significance of differences in alanine and taurine between the 5-FU- and saline-administered groups was determined only after recovery correction (0.75 +/- 0.12 versus 0.86 +/- 0.07 for alanine; 5.17 +/- 0.59 versus 5.66 +/- 0.42 for taurine [mu mol/g brain tissue]; P < 0.05).Conclusion: A new recovery correction technique corrected metabolite loss during PCA extraction, minimized inter-assay variance in quantitative 1H NMR spectroscopy of brain tissue, and effectively detected inter-group differences in concentrations of brain metabolites between 5-FU- and saline-administered rats.
- リンク情報
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- DOI
- https://doi.org/10.2463/mrms.2013-0089
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/24990468
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000342275600001&DestApp=WOS_CPL
- Jamas Url
- https://search.jamas.or.jp/index.php?module=Default&action=Link&doc_id=20140929450001&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F24990468&type=PubMed&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00001_1.gif
- Jamas Url
- https://search.jamas.or.jp/index.php?module=Default&action=Link&doc_id=20140929450001&url=http%3A%2F%2Fci.nii.ac.jp%2Fnaid%2F130004470999&type=CiNii&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00003_1.gif
- Jamas Url
- https://search.jamas.or.jp/index.php?module=Default&action=Link&doc_id=20140929450001&url=https%3A%2F%2Fdoi.org%2F10.2463%2Fmrms.2013-0089&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif
- ID情報
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- DOI : 10.2463/mrms.2013-0089
- ISSN : 1347-3182
- eISSN : 1880-2206
- 医中誌Web ID : 2015134669
- PubMed ID : 24990468
- Web of Science ID : WOS:000342275600001