論文

査読有り
2016年5月

Origins of oligodendrocytes in the cerebellum, whose development is controlled by the transcription factor, Sox9

MECHANISMS OF DEVELOPMENT
  • Ryoya Hashimoto
  • ,
  • Kei Hori
  • ,
  • Tomoo Owa
  • ,
  • Satoshi Miyashita
  • ,
  • Kenichi Dewa
  • ,
  • Norihisa Masuyama
  • ,
  • Kazuhisa Sakai
  • ,
  • Yoneko Hayase
  • ,
  • Yusuke Seto
  • ,
  • Yukiko U. Inoue
  • ,
  • Takayoshi Inoue
  • ,
  • Noritaka Ichinohe
  • ,
  • Yoshiya Kawaguchi
  • ,
  • Haruhiko Akiyama
  • ,
  • Schuichi Koizumi
  • ,
  • Mikio Hoshino

140
開始ページ
25
終了ページ
40
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.mod.2016.02.004
出版者・発行元
ELSEVIER SCIENCE BV

Development of oligodendrocytes, myelin-forming glia in the central nervous system (CNS), proceeds on a protracted schedule. Specification of oligodendrocyte progenitor cells (OPCs) begins early in development, whereas their terminal differentiation occurs at late embryonic and postnatal periods. However, for oligodendrocytes in the cerebellum, the developmental origins and the molecular machinery to control these distinct steps remain unclear. By in vivo fate mapping and immunohistochemical analyses, we obtained evidence that the majority of oligodendrocytes in the cerebellum originate from the Olig2-expressing neuroepithelial domain in the ventral rhombomere 1 (r1), while about 6% of cerebellar oligodendrocytes are produced in the cerebellar ventricular zone. Furthermore, to elucidate the molecular determinants that regulate their development, we analyzed mice in which the transcription factor Sox9 was specifically ablated from the cerebellum, ventral r1 and caudal midbrain by means of the Cre/loxP recombination system. This resulted in a delay in the birth of OPCs and subsequent developmental aberrations in these cells in the Sox9-deficient mice. In addition, we observed altered proliferation of OPCs, resulting in a decrease in oligodendrocyte numbers that accompanied an attenuation of the differentiation and an increased rate of apoptosis. Results from in vitro assays using oligodendrocyte-enriched cultures further supported our observations from in vivo experiments. These data suggest that Sox9 participates in the development of oligodendrocytes in the cerebellum, by regulating the timing of their generation, proliferation, differentiation and survival. (C) 2016 Elsevier Ireland Ltd. All rights reserved.

Web of Science ® 被引用回数 : 12

リンク情報
DOI
https://doi.org/10.1016/j.mod.2016.02.004
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000376545000004&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.mod.2016.02.004
  • ISSN : 0925-4773
  • eISSN : 1872-6356
  • Web of Science ID : WOS:000376545000004

エクスポート
BibTeX RIS