論文

査読有り 国際誌
2019年7月

Effect of the neuropeptide phoenixin and its receptor GPR173 during folliculogenesis.

Reproduction (Cambridge, England)
  • Xuan Phuoc Nguyen
  • Tomoko Nakamura
  • Satoko Osuka
  • Bayasula Bayasula
  • Natsuki Nakanishi
  • Yukiyo Kasahara
  • Ayako Muraoka
  • Shotaro Hayashi
  • Takashi Nagai
  • Tomohiko Murase
  • Maki Goto
  • Akira Iwase
  • Fumitaka Kikkawa
  • 全て表示

158
1
開始ページ
25
終了ページ
34
記述言語
英語
掲載種別
DOI
10.1530/REP-19-0025

Folliculogenesis is a complex process, defined by the growth and development of follicles from the primordial population. Granulosa cells (GCs) play a vital role in every stage of follicular growth through proliferation, acquisition of gonadotropic responsiveness, steroidogenesis and production of autocrine/paracrine factors. A recently discovered hypothalamic neuropeptide phoenixin is involved in the regulation of the reproductive system. Phoenixin acts through its receptor, G protein-coupled receptor 173 (GPR173), to activate the cAMP/PKA pathway leading to the phosphorylation of CREB (pCREB). Here, we demonstrated the expression patterns of phoenixin and GPR173 in human ovary and explored its role in folliculogenesis. Phoenixin and GPR173 were both expressed in the human ovarian follicle, with increased expression in GCs as the follicle grows. Phoenixin treatment at 100 nM for 24 h induced the proliferation of human non-luteinized granulosa cell line, HGrC1 and significantly increased the expression levels of CYP19A1, FSHR, LHR and KITL, but decreased NPPC expression levels. These effects were suppressed by GPR173 siRNA. The expression level of CREB1, pCREB and estradiol (E2) production in the culture medium was significantly enhanced by phoenixin treatment in a concentration-dependent manner. Phoenixin also significantly increased the follicular area in a murine ovarian tissue culture model, leading to an increased number of ovulated oocytes with a higher level of maturation. Taken together, our data demonstrate that phoenixin is an intraovarian factor that promotes follicular growth through its receptor GPR173 by accelerating proliferation of GCs, inducing E2 production and increasing the expression of genes related to follicle development.

リンク情報
DOI
https://doi.org/10.1530/REP-19-0025
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30933929
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000466887200006&DestApp=WOS_CPL
ID情報
  • DOI : 10.1530/REP-19-0025
  • ISSN : 1470-1626
  • PubMed ID : 30933929
  • Web of Science ID : WOS:000466887200006

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