論文

査読有り
2007年11月

Tom20 recognizes mitochondrial presequences through dynamic equilibrium among multiple bound states

EMBO JOURNAL
  • Takashi Saitoh
  • ,
  • Mayumi Igura
  • ,
  • Takayuki Obita
  • ,
  • Toyoyuki Ose
  • ,
  • Rieko Kojima
  • ,
  • Katsumi Maenaka
  • ,
  • Toshiya Endo
  • ,
  • Daisuke Kohda

26
22
開始ページ
4777
終了ページ
4787
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/sj.emboj.7601888
出版者・発行元
NATURE PUBLISHING GROUP

Most mitochondrial proteins are synthesized in the cytosol and imported into mitochondria. The N-terminal presequences of mitochondrial-precursor proteins contain a diverse consensus motif (phi chi chi phi phi, phi is hydrophobic and chi is any amino acid), which is recognized by the Tom20 protein on the mitochondrial surface. To reveal the structural basis of the broad selectivity of Tom20, the Tom20 presequence complex was crystallized. Tethering a presequence peptide to Tom20 through a disulfide bond was essential for crystallization. Unexpectedly, the two crystals with different linker designs provided unique relative orientations of the presequence with respect to Tom20, and neither configuration could fully account for the hydrophobic preference at the three hydrophobic positions of the consensus motif. We propose the existence of a dynamic equilibrium in solution among multiple states including the two bound states. In accordance, NMR N-15 relaxation analyses suggested motion on a sub-millisecond timescale at the Tom20-presequence interface. We suggest that the dynamic, multiple-mode interaction is the molecular mechanism facilitating the broadly selective specificity of the Tom20 receptor toward diverse mitochondrial presequences.

Web of Science ® 被引用回数 : 98

リンク情報
DOI
https://doi.org/10.1038/sj.emboj.7601888
CiNii Articles
http://ci.nii.ac.jp/naid/80018052247
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/17948058
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000250966800015&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/sj.emboj.7601888
  • ISSN : 0261-4189
  • CiNii Articles ID : 80018052247
  • PubMed ID : 17948058
  • Web of Science ID : WOS:000250966800015

エクスポート
BibTeX RIS