論文

査読有り
2007年12月

Molecular interaction of ferredoxin and ferredoxin-NADP(+) reductase from human malaria parasite

JOURNAL OF BIOCHEMISTRY
  • Yoko Kimata-Ariga
  • ,
  • Takashi Saitoh
  • ,
  • Takahisa Ikegami
  • ,
  • Toshihiro Horii
  • ,
  • Toshiharu Hasel

142
6
開始ページ
715
終了ページ
720
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/jb/mvm184
出版者・発行元
OXFORD UNIV PRESS

The malaria parasite possesses plant-type ferredoxin (Fd) and ferredoxin-NADP(+) reductase (FNR) in a plastid-derived organelle called the apicoplast. This Fd/FNR redox system, which potentially provides reducing power for essential biosynthetic pathways in the apicoplast, has been proposed as a target for the development of specific new anti-malarial agents. We studied the molecular interaction of Fd and FNR of human malaria parasite (Plasmodium falciparum), which were produced as recombinant proteins in Escherichia coli. NMR chemical shift perturbation analysis mapped the location of the possible FNR interaction sites on the surface of P. falciparum Fd. Site-specific mutation of acidic Fd residues in these regions and the resulting analyses of electron transfer activity and affinity chromatography of those mutants revealed that two acidic regions (a region including Asp26, Glu29 and Glu34, and the other including Asp65 and Glu66) dominantly contribute to the electrostatic interaction with P. falciparum FNR. The combination of Asp26/Glu29/Glu34 conferred a larger contribution than that of Asp65/Glu66, and among Asp26, Glu29 and Glu34, Glu29 was. shown to be the most important residue for the interaction with P. falciparum FNR. These findings provide the basis for understanding molecular recognition between Fd and FNR of the malaria parasite.

Web of Science ® 被引用回数 : 13

リンク情報
DOI
https://doi.org/10.1093/jb/mvm184
CiNii Articles
http://ci.nii.ac.jp/naid/10020095205
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/17938142
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000254996700007&DestApp=WOS_CPL
ID情報
  • DOI : 10.1093/jb/mvm184
  • ISSN : 0021-924X
  • CiNii Articles ID : 10020095205
  • PubMed ID : 17938142
  • Web of Science ID : WOS:000254996700007

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