論文

査読有り
2017年5月

Dissociation of Per1 and Bmal1 circadian rhythms in the suprachiasmatic nucleus in parallel with behavioral outputs

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
  • Daisuke Ono
  • ,
  • Sato Honma
  • ,
  • Yoshihiro Nakajima
  • ,
  • Shigeru Kuroda
  • ,
  • Ryosuke Enoki
  • ,
  • Ken-ichi Honma

114
18
開始ページ
E3699
終了ページ
E3708
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1073/pnas.1613374114
出版者・発行元
NATL ACAD SCIENCES

The temporal order of physiology and behavior in mammals is primarily regulated by the circadian pacemaker located in the hypothalamic suprachiasmatic nucleus (SCN). Taking advantage of bioluminescence reporters, we monitored the circadian rhythms of the expression of clock genes Per1 and Bmal1 in the SCN of freely moving mice and found that the rate of phase shifts induced by a single light pulse was different in the two rhythms. The Per1-luc rhythm was phase-delayed instantaneously by the light presented at the subjective evening in parallel with the activity onset of behavioral rhythm, whereas the Bmal1-ELuc rhythm was phasedelayed gradually, similar to the activity offset. The dissociation was confirmed in cultured SCN slices of mice carrying both Per1-luc and Bmal1-ELuc reporters. The two rhythms in a single SCN slice showed significantly different periods in a long-term (3 wk) culture and were internally desynchronized. Regional specificity in the SCN was not detected for the period of Per1-luc and Bmal1-ELuc rhythms. Furthermore, neither is synchronized with circadian intracellular Ca2+ rhythms monitored by a calcium indicator, GCaMP6s, or with firing rhythms monitored on a multielectrode array dish, although the coupling between the circadian firing and Ca2+ rhythms persisted during culture. These findings indicate that the expressions of two key clock genes, Per1 and Bmal1, in the SCN are regulated in such a way that they may adopt different phases and free-running periods relative to each other and are respectively associated with the expression of activity onset and offset.

リンク情報
DOI
https://doi.org/10.1073/pnas.1613374114
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28416676
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000400358000015&DestApp=WOS_CPL
ID情報
  • DOI : 10.1073/pnas.1613374114
  • ISSN : 0027-8424
  • PubMed ID : 28416676
  • Web of Science ID : WOS:000400358000015

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