2020年7月14日
An Artificial Conversion of Roots into Organs with Shoot Stem Characteristics by Inducing Two Transcription Factors
iScience
- 巻
- 23
- 号
- 7
- 開始ページ
- 101332
- 終了ページ
- 101332
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.isci.2020.101332
- 出版者・発行元
- Elsevier BV
Somatic plant cells can regenerate shoots and/or roots or adventitious embryonic calluses, which may induce organ formation under certain conditions. Such regenerations occur via dedifferentiation of somatic cells, induction of organs, and their subsequent outgrowth. Despite recent advances in understanding of plant regeneration, many details of shoot induction remain unclear. Here, we artificially induced shoot stem-like green organs (SSOs) in Arabidopsis thaliana roots via simultaneous induction of two transcription factors (TFs), ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 25 (ATHB25, At5g65410) and the B3 family transcription factor REPRODUCTIVE MERISTEM 7 (REM7, At3g18960). The SSOs exhibited negative gravitropism and differentiated vascular bundle phenotypes. The ATHB25/REM7 induced the expression of genes controlling shoot stem characteristics by ectopic expression in roots. Intriguingly, the restoration of root growth was seen in the consecutive and adjacent parts of the SSOs under gene induction conditions. Our findings thus provide insights into the development and regeneration of plant shoot stems.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.isci.2020.101332
- ISSN : 2589-0042
- ORCIDのPut Code : 77296120
- PubMed ID : 32668199
- PubMed Central 記事ID : PMC7385925