2017年1月
Neuropeptide Y Y-5 receptor localization in mouse central nervous system
BRAIN RESEARCH
- ,
- ,
- 巻
- 1655
- 号
- 開始ページ
- 216
- 終了ページ
- 232
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.brainres.2016.10.026
- 出版者・発行元
- ELSEVIER SCIENCE BV
Neuropeptide Y (NPY) and its receptors affect blood pressure, feeding behavior, and neurogenesis. In this study, the distribution of neurons expressing NPY Y-5 receptor (Y-5) was examined in adult mouse central nervous system by immunohistochemistry. Y-5 protein localization was investigated using polyclonal anti-Y-5 antibody, which was successfully preabsorbed with Y-5 knockout brain tissues. The preabsorbed anti-Y-5 antibody did not react with Y-5 knockout brain tissues, thus meeting the "hard specificity criterion," which is the absence of staining in tissues genetically deficient for the antigen (Pradidarcheep et al., 2008). Y-5-positive neurons were found in most brain areas. Most Y-5 immunoreactivities were observed as dot-like structures adjacent to the plasma membrane, as expected for a cell membrane receptor. In situ hybridization showed that the Y-5 mRNA expression was correlated with the Y-5 protein level in each case and that it was probably controlled by the transcriptional regulation of the Y-5 gene. In the nuclei where Y-5 was expressed, Y-5 immunoreactivities were found mainly in the somatic and dendritic areas. The distribution patterns of the Y-5-positive cells that were broader than previously expected suggest important biological activities of the Y-5 in many brain areas.
- リンク情報
- ID情報
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- DOI : 10.1016/j.brainres.2016.10.026
- ISSN : 0006-8993
- eISSN : 1872-6240
- PubMed ID : 27984021
- Web of Science ID : WOS:000392775300025