論文

査読有り
2017年1月

Neuropeptide Y Y-5 receptor localization in mouse central nervous system

BRAIN RESEARCH
  • Shin-ichi Murase
  • ,
  • Tomohiro Shiiya
  • ,
  • Hiroshi Higuchi

1655
開始ページ
216
終了ページ
232
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.brainres.2016.10.026
出版者・発行元
ELSEVIER SCIENCE BV

Neuropeptide Y (NPY) and its receptors affect blood pressure, feeding behavior, and neurogenesis. In this study, the distribution of neurons expressing NPY Y-5 receptor (Y-5) was examined in adult mouse central nervous system by immunohistochemistry. Y-5 protein localization was investigated using polyclonal anti-Y-5 antibody, which was successfully preabsorbed with Y-5 knockout brain tissues. The preabsorbed anti-Y-5 antibody did not react with Y-5 knockout brain tissues, thus meeting the "hard specificity criterion," which is the absence of staining in tissues genetically deficient for the antigen (Pradidarcheep et al., 2008). Y-5-positive neurons were found in most brain areas. Most Y-5 immunoreactivities were observed as dot-like structures adjacent to the plasma membrane, as expected for a cell membrane receptor. In situ hybridization showed that the Y-5 mRNA expression was correlated with the Y-5 protein level in each case and that it was probably controlled by the transcriptional regulation of the Y-5 gene. In the nuclei where Y-5 was expressed, Y-5 immunoreactivities were found mainly in the somatic and dendritic areas. The distribution patterns of the Y-5-positive cells that were broader than previously expected suggest important biological activities of the Y-5 in many brain areas.

リンク情報
DOI
https://doi.org/10.1016/j.brainres.2016.10.026
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27984021
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000392775300025&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.brainres.2016.10.026
  • ISSN : 0006-8993
  • eISSN : 1872-6240
  • PubMed ID : 27984021
  • Web of Science ID : WOS:000392775300025

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