論文

査読有り 国際誌
2010年4月13日

Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells.

Proceedings of the National Academy of Sciences of the United States of America
  • Haruko Ueda
  • ,
  • Etsuo Yokota
  • ,
  • Natsumaro Kutsuna
  • ,
  • Tomoo Shimada
  • ,
  • Kentaro Tamura
  • ,
  • Teruo Shimmen
  • ,
  • Seiichiro Hasezawa
  • ,
  • Valerian V Dolja
  • ,
  • Ikuko Hara-Nishimura

107
15
開始ページ
6894
終了ページ
9
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1073/pnas.0911482107
出版者・発行元
NATL ACAD SCIENCES

Plants exhibit an ultimate case of the intracellular motility involving rapid organelle trafficking and continuous streaming of the endoplasmic reticulum (ER). Although it was long assumed that the ER dynamics is actomyosin-driven, the responsible myosins were not identified, and the ER streaming was not characterized quantitatively. Here we developed software to generate a detailed velocity-distribution map for the GFP-labeled ER. This map revealed that the ER in the most peripheral plane was relatively static, whereas the ER in the inner plane was rapidly streaming with the velocities of up to approximately 3.5 microm/sec. Similar patterns were observed when the cytosolic GFP was used to evaluate the cytoplasmic streaming. Using gene knockouts, we demonstrate that the ER dynamics is driven primarily by the ER-associated myosin XI-K, a member of a plant-specific myosin class XI. Furthermore, we show that the myosin XI deficiency affects organization of the ER network and orientation of the actin filament bundles. Collectively, our findings suggest a model whereby dynamic three-way interactions between ER, F-actin, and myosins determine the architecture and movement patterns of the ER strands, and cause cytosol hauling traditionally defined as cytoplasmic streaming.

リンク情報
DOI
https://doi.org/10.1073/pnas.0911482107
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/20351265
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2872430
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000276642100061&DestApp=WOS_CPL
ID情報
  • DOI : 10.1073/pnas.0911482107
  • ISSN : 0027-8424
  • PubMed ID : 20351265
  • PubMed Central 記事ID : PMC2872430
  • Web of Science ID : WOS:000276642100061

エクスポート
BibTeX RIS