2017年
Isolation of protein storage vacuoles and their membranes
Methods in Molecular Biology
- ,
- 巻
- 1511
- 号
- 開始ページ
- 163
- 終了ページ
- 168
- 記述言語
- 英語
- 掲載種別
- 論文集(書籍)内論文
- DOI
- 10.1007/978-1-4939-6533-5_13
- 出版者・発行元
- Humana Press Inc.
Protein-storage vacuoles (PSVs) are specialized vacuoles that sequester large amounts of storage proteins. During seed development, PSVs are formed de novo and/or from preexisting lytic vacuoles. Seed PSVs can be subdivided into four distinct compartments: membrane, globoid, matrix, and crystalloid. In this chapter, we introduce easy methods for isolation of PSVs and their membranes from pumpkin seeds. These methods facilitate the identification and characterization of PSV components.
- リンク情報
- ID情報
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- DOI : 10.1007/978-1-4939-6533-5_13
- ISSN : 1064-3745
- eISSN : 1940-6029
- PubMed ID : 27730610
- SCOPUS ID : 84991467011