2000年
Characterization of organelles in the vacuolar-sorting pathway by visualization with GFP in tobacco BY-2 cells
Plant and Cell Physiology
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- 巻
- 41
- 号
- 9
- 開始ページ
- 993
- 終了ページ
- 1001
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/pcp/pcd040
- 出版者・発行元
- Japanese Society of Plant Physiologists
We have shown the localization and mobilization of modified green fluorescent proteins (GFPs) with various signals in different compartments in a vacuolar-sorting system of tobacco BY-2 cells. In contrast to the efficient secretion of GFP from the transformed cells expressing SP-GFP composed of a signal peptide and GFP, accumulation of GFP in the vacuoles was observed in the cells expressing SP-GFP fused with the C-terminal peptide of pumpkin 2S albumin. This indicated that this peptide is sufficient for vacuolar targeting. Interestingly, the fluorescence in the vacuoles disappeared sharply at 7 d after inoculation of the cells, but it appeared again after re-inoculation into a new culture medium. When SP-GFP was fused with the region, termed PV72C, including a transmembrane domain and a cytosolic tail of a vacuolar-sorting receptor PV72, GFP-PV72C was detected in the Golgi-complex-like small particles. Prolonged culture showed that GFP-PV72C that reached the prevacuolar compartments was cleaved off the PV72C region to produce GFP, that arrived at the vacuoles to be diffused. These findings suggested that the vacuolar-sorting receptor might be recycled between the Golgi complex and prevacuolar compartments.
- リンク情報
- ID情報
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- DOI : 10.1093/pcp/pcd040
- ISSN : 0032-0781
- CiNii Articles ID : 10004603770
- PubMed ID : 11100771
- SCOPUS ID : 0033780821