論文

査読有り
2017年8月

Foxc2 influences alveolar epithelial cell differentiation during lung development

DEVELOPMENT GROWTH & DIFFERENTIATION
  • Mayoko Tsuji
  • ,
  • Masae Morishima
  • ,
  • Kazuhiko Shimizu
  • ,
  • Shunichi Morikawa
  • ,
  • Mikael Heglind
  • ,
  • Sven Enerback
  • ,
  • Taichi Ezaki
  • ,
  • Jun Tamaoki

59
6
開始ページ
501
終了ページ
514
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/dgd.12368
出版者・発行元
WILEY

FOXC2, a forkhead transcriptional factor, is a candidate gene for congenital heart diseases and lymphedema-distichiasis syndrome and yellow nail syndrome; however, there are no reports on Foxc2 and the development of the lung. We have identified lung abnormalities in Foxc2-knockout embryos during investigation of cardiac development. The aim of this study was to clarify the morphological characteristics during lung development using ICR-Foxc2 knockout lungs. Mutant fetuses at embryonic days 10.5-18.5 were obtained from mating of Foxc2(+/-) mice and then analyzed. Notably, Foxc2-knockout lungs appeared parenchymatous and much smaller than those of the wild-type littermates. In the Foxc2 knockout lungs, the capillary beds remained distant from the alveolar epithelium until the late stages, the number of type2 alveolar cells per alveolar progenitor cell was lower and the type1 alveolar cells were thicker in Foxc2 knockout mice. In contrast, Foxc2 expression was only detected in the mesenchyme of the lung buds at E10.5, and it disappeared at E11.5 in Foxc2-LacZ knockin mice. Furthermore, the expression of Lef1 was significantly inhibited in E11.5 lungs. All of these results suggest that the abnormalities in Foxc2 knockout mice may involve maldifferentiation of alveolar epithelial cells and capillary vessel endothelial-alveolar epithelial approach as well as lymph vessel malformation. This is the first report about relationship between Foxc2 and lung development. This animal model might provide an important clue for elucidating the mechanism of lung development and the cause of respiratory diseases.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.1111/dgd.12368
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000408835800002&DestApp=WOS_CPL

エクスポート
BibTeX RIS