論文

査読有り
2016年7月

Development and evaluation of an interferon-gamma release assay in Asian elephants (Elephas maximus)

JOURNAL OF VETERINARY MEDICAL SCIENCE
  • Sarad Paudel
  • ,
  • Marvin A. Villanueva
  • ,
  • Susan K. Mikota
  • ,
  • Chie Nakajima
  • ,
  • Kamal P. Gairhe
  • ,
  • Suraj Subedi
  • ,
  • Nabin Rayamajhi
  • ,
  • Mariko Sashika
  • ,
  • Michito Shimozuru
  • ,
  • Takashi Matsuba
  • ,
  • Yasuhiko Suzuki
  • ,
  • Toshio Tsubota

78
7
開始ページ
1117
終了ページ
1121
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1292/jvms.15-0701
出版者・発行元
JAPAN SOC VET SCI

We developed an interferon-gamma release assay (IGRA) specific for Asian elephants (Elephas maximus). Whole blood collected from forty captive Asian elephants was stimulated with three different mitogens i.e., phytohemagglutinin (PHA), pokweed mitogen (PWM) and phorbol myristate aceteate/ionomycin (PMA/I). A sandwich ELISA that was able to recognize the recombinant elephant interferon-gamma (rEIFN-gamma) as well as native interferon-gamma from the Asian elephants was performed using anti-elephant IFN-gamma rabbit polyclonal antibodies as capture antibodies and biotinylated anti-elephant IFN-gamma rabbit polyclonal antibodies as detection antibodies. PMA/I was the best mitogen to use as a positive control for an Asian elephant IGRA. The development of an Asian elephant-specific IGRA that detects native IFN-gamma in elephant whole blood provides promising results for its application as a potential diagnostic tool for diseases, such as tuberculosis (TB) in Asian elephants.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.1292/jvms.15-0701
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000380622000004&DestApp=WOS_CPL