論文

査読有り 国際誌
2019年12月

PMab-241 Specifically Detects Bear Podoplanin of Lymphatic Endothelial Cells in the Lung of Brown Bear.

Monoclonal antibodies in immunodiagnosis and immunotherapy
  • Junko Takei
  • ,
  • Shinji Yamada
  • ,
  • Satoru Konnai
  • ,
  • Tsuyoshi Ishinazaka
  • ,
  • Michito Shimozuru
  • ,
  • Mika K Kaneko
  • ,
  • Yukinari Kato

38
6
開始ページ
282
終了ページ
284
記述言語
英語
掲載種別
DOI
10.1089/mab.2019.0038

Podoplanin (PDPN)/T1alpha is utilized as a specific marker of lymphatic endothelial cells or type I alveolar cells of lung. Therefore, sensitive and specific monoclonal antibodies (mAbs) detecting PDPN are necessary for immunohistochemical analyses, especially using formalin-fixed paraffin-embedded tissues. Recently, we developed an anti-bear PDPN (bPDPN) mAb, PMab-247, which is useful for immunohistochemical analyses to detect both lymphatic endothelial cells and type I alveolar cells of lung. However, it is difficult to distinguish lymphatic endothelial cells from type I alveolar cells in the bear lung. In this study, we showed that a novel anti-bPDPN mAb, PMab-241 stained only lymphatic endothelial cells, not type I alveolar cells of the lung in immunohistochemical analyses. These findings suggest that PMab-241 could be useful for staining lymphatic endothelial cells specifically in the bear lung tissues.

リンク情報
DOI
https://doi.org/10.1089/mab.2019.0038
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31721640