2010年9月
The Matrix Protein hnRNP U Is Required for Chromosomal Localization of Xist RNA
DEVELOPMENTAL CELL
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- 巻
- 19
- 号
- 3
- 開始ページ
- 469
- 終了ページ
- 476
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.devcel.2010.08.006
- 出版者・発行元
- CELL PRESS
In XX female mammals, one of the two X chromosomes is epigenetically inactivated to equalize gene expression with XY males. The formation of the inactive X chromosome (Xi) is regulated by an X-linked long noncoding RNA Xist, which accumulates on the entire length of the chromosome in cis and induces heterochromatin formation. However, the mechanism by which Xist RNA "paints" the Xi has long remained elusive. Here, we show that a matrix protein hnRNP U/SP120/SAF-A is required for the accumulation of Xist RNA on the Xi. Xist RNA and hnRNP U interact and upon depletion of hnRNP U, Xist RNA is detached from the Xi and diffusely localized into the nucleoplasm. In addition, ES cells lacking hnRNP U expression fail to form the Xi. We propose that the association with matrix proteins is an essential step in the epigenetic regulation of gene expression by Xist RNA.
- リンク情報
- ID情報
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- DOI : 10.1016/j.devcel.2010.08.006
- ISSN : 1534-5807
- PubMed ID : 20833368
- Web of Science ID : WOS:000282203100015