Papers

Peer-reviewed
12 2014

Effects of protein tyrosine phosphatase-PEST are reversed by Akt in T cells

CELLULAR SIGNALLING
  • Yutaka Arimura
  • ,
  • Kazuhiko Shimizu
  • ,
  • Madoka Koyanagi
  • ,
  • Junji Yagi

Volume
26
Number
12
First page
2721
Last page
2729
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1016/j.cellsig.2014.08.014
Publisher
ELSEVIER SCIENCE INC

T cell activation is regulated by a balance between phosphorylation and dephosphorylation that is under the control of kinases and phosphatases. Here, we examined the role of a non-receptor-type protein tyrosine phosphatase, PTP-PEST, using retrovirus-mediated gene transduction into murine T cells. Based on observations of vector markers (GFP or Thy1.1), exogenous PTP-PEST-positive CD4(+) T cells appeared within 2 days after gene transduction; the percentage of PIP-PEST-positive cells tended to decrease during a resting period in the presence of IL-2 over the next 2 days. These vector markers also showed much lower expression intensities, compared with control cells, suggesting a correlation between the percent reduction and the low marker expression intensity. A catalytically inactive PIP-PEST mutant also showed the same tendency, and stepwise deletion mutants gradually lost their ability to induce the above phenomenon. On the other hand, these PIP-PEST-transduced cells did not have an apoptotic phenotype. No difference in the total cell numbers was found in the wells of a culture plate containing VEC- and PIP-PEST-transduced T cells. Moreover, serine/threonine kinase Akt, but not the anti-apoptotic molecules Bc1-2 and Bcl-XL, reversed the phenotype induced by PTP-PEST. We discuss the novel mechanism by which Ala interferes with PIP-PEST. (C) 2014 Elsevier Inc. All rights reserved.

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Link information
DOI
https://doi.org/10.1016/j.cellsig.2014.08.014
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25152368
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000345107700015&DestApp=WOS_CPL