論文

査読有り
2007年

Quantum dot-doped silica nanoparticles as probes for targeting of T-lymphocytes

INTERNATIONAL JOURNAL OF NANOMEDICINE
  • Massimo Bottini
  • ,
  • Federica D'Annibale
  • ,
  • Andrea Magrini
  • ,
  • Fabio Cerignoli
  • ,
  • Yutaka Arimura
  • ,
  • Marcia I. Dawson
  • ,
  • Enrico Bergamaschi
  • ,
  • Nicola Rosato
  • ,
  • Antonio Bergamaschi
  • ,
  • Tomas Mustelin

2
2
開始ページ
227
終了ページ
233
記述言語
英語
掲載種別
研究論文(学術雑誌)
出版者・発行元
DOVE MEDICAL PRESS LTD

To enhance diagnostic or therapeutic efficacy, novel nanomaterials must be engineered to function in biologically relevant environments, be visible by conventional fluorescent microscopy, and have multivalent loading capacity for easy detection or effective drug delivery. Here we report the fabrication of silica nanoparticles doped with quantum dots and superficially functionalized with amino and phosphonate groups. The amino groups were acylated with a water-soluble biotin-labeling reagent. The biotinylated nanoparticles were subsequently decorated with neutravidin by exploiting the strong affinity between neutravidin and biotin. The resultant neutravidin-decorated fluorescent silica nanoparticles stably dispersed under physiological conditions, were visible by conventional optical and confocal fluorescent microscopy, and could be further functionalized with macromolecules, nucleic acids, and polymers. We also coated the surface of the nanoparticles with biotinylated mouse anti-human CD3 (alpha CD3). The resultant fluorescent nanoassembly was taken up by Jurkat T cells through receptor-mediated endocytosis and was partially released to lysosomes. Thus, quantum dot-doped silica nanoparticles decorated with neutravidin represent a potentially excellent scaffold for constructing specific intracellular nanoprobes and transporters.

Web of Science ® 被引用回数 : 66

リンク情報
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/17722550
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000252051600010&DestApp=WOS_CPL

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