論文

査読有り
2003年7月

Regulatory roles of IL-2 and IL-4 in H4/inducible costimulator expression on activated CD4(+) T cells during Th cell development

JOURNAL OF IMMUNOLOGY
  • J Yagi
  • ,
  • Y Arimura
  • ,
  • U Dianzani
  • ,
  • T Uede
  • ,
  • T Okamoto
  • ,
  • T Uchiyama

171
2
開始ページ
783
終了ページ
794
記述言語
英語
掲載種別
研究論文(学術雑誌)
出版者・発行元
AMER ASSOC IMMUNOLOGISTS

We found a tight correlation among the levels of H4/inducible costimulator (ICOS) expression, IL-4 production, and GATA-3 induction, using activated CD4(+) T cells obtained from six different murine strains. BALB/c-activated CD4(+) T cells expressed similar to10-fold more H4/ICOS on their surfaces and produced similar to10-fold more IL-4 upon restimulation than C57BL/6-activated CD4(+) T cells. BALB/c naive CD4(+) T cells were shown to produce much higher amounts of IL-2 and IL-4 upon primary stimulation than C57BL/6 naive CD4(+) T cells. Neutralization of IL-4 with mAbs in culture of BALB/c naive CD4(+) T cells strongly down-regulated both H4/ICOS expression on activated CD4(+) T cells and IL-4 production upon subsequent restimulation. Conversely, exogenous IL-4 added to the culture of BALB/c or C57BL/6 naive CD4(+) T cells up-regulated H4/ICOS expression and IL-4 production upon restimulation. In addition, retroviral expression of GATA-3 during the stimulation of naive CD4(+) T cells from C57BL/6 or IL-4(-/-) mice increased H4/ICOS expression on activated CD4(+) T cells. A similar effect of IL-2 in the primary culture of BALB/c naive CD4(+) T cells appeared to be mediated by IL-4, the production of which was regulated by IL-2. These data suggest that IL-4 induced by IL-2 is critical to the maintenance of high H4/ICOS expression on BALB/c-activated CD4(+) T cells.

Web of Science ® 被引用回数 : 27

リンク情報
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/12847246
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000184093800037&DestApp=WOS_CPL