論文

査読有り
2016年2月

Control of enzymatic degradation of biodegradable polymers by treatment with biosurfactants, mannosylerythritol lipids, derived from Pseudozyma spp. yeast strains

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Tokuma Fukuoka
  • ,
  • Yukiko Shinozaki
  • ,
  • Wataru Tsuchiya
  • ,
  • Ken Suzuki
  • ,
  • Takashi Watanabe
  • ,
  • Toshimasa Yamazaki
  • ,
  • Dai Kitamoto
  • ,
  • Hiroko Kitamoto

100
4
開始ページ
1733
終了ページ
1741
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00253-015-7068-1
出版者・発行元
SPRINGER

Cutinase-like esterase from the yeasts Pseudozyma antarctica (PaE) shows strong degradation activity in an agricultural biodegradable plastic (BP) model of mulch films composed of poly(butylene succinate-co-adipate) (PBSA). P. antarctica is known to abundantly produce a glycolipid biosurfactant, mannosylerythritol lipid (MEL). Here, the effects of MEL on PaE-catalyzed degradation of BPs were investigated. Based on PBSA dispersion solution, the degradation of PBSA particles by PaE was inhibited in the presence of MEL. MEL behavior on BP substrates was monitored by surface plasmon resonance (SPR) using a sensor chip coated with polymer films. The positive SPR signal shift indicated that MEL readily adsorbed and spread onto the surface of a BP film. The amount of BP degradation by PaE was monitored based on the negative SPR signal shift and was decreased 1.7-fold by MEL pretreatment. Furthermore, the shape of PBSA mulch films in PaE-containing solution was maintained with MEL pretreatment, whereas untreated films were almost completely degraded and dissolved. These results suggest that MEL covering the surface of BP film inhibits adsorption of PaE and PaE-catalyzed degradation of BPs. We applied the above results to control the microbial degradation of BP mulch films. MEL pretreatment significantly inhibited BP mulch film degradation by both PaE solution and BP-degradable microorganism. Moreover, the degradation of these films was recovered after removal of the coated MEL by ethanol treatment. These results demonstrate that the biodegradation of BP films can be readily and reversibly controlled by a physical approach using MEL.

Web of Science ® 被引用回数 : 9

リンク情報
DOI
https://doi.org/10.1007/s00253-015-7068-1
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26512003
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000369309000017&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00253-015-7068-1
  • ISSN : 0175-7598
  • eISSN : 1432-0614
  • PubMed ID : 26512003
  • Web of Science ID : WOS:000369309000017

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