論文

査読有り
2014年7月

Mannosylerythritol lipids secreted by phyllosphere yeast Pseudozyma antarctica is associated with its filamentous growth and propagation on plant surfaces

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Shigenobu Yoshida
  • ,
  • Tomotake Morita
  • ,
  • Yukiko Shinozaki
  • ,
  • Takashi Watanabe
  • ,
  • Yuka Sameshima-Yamashita
  • ,
  • Motoo Koitabashi
  • ,
  • Dai Kitamoto
  • ,
  • Hiroko Kitamoto

98
14
開始ページ
6419
終了ページ
6429
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00253-014-5675-x
出版者・発行元
SPRINGER

The biological function of mannosylerythritol lipids (MELs) towards their producer, Pseudozyma antarctica, on plant surfaces was investigated. MEL-producing wild-type strain and its MEL production-defective mutant strain (Delta PaEMT1) were compared in terms of their phenotypic traits on the surface of plastic plates, onion peels, and fresh leaves of rice and wheat. While wild-type cells adhering on plastic surfaces and onion peels changed morphologically from single cells to elongated ones for a short period of about 4 h and 1 day, respectively, Delta PaEMT1 cells did not. Microscopic observation of both strains grown on plant leaf surfaces verified that the wild type colonized a significantly bigger area than that of Delta PaEMT1. However, when MELs were exogenously added to the mutant cells on plant surfaces, their colonized area became enlarged. High-performance liquid chromatography analysis revealed a secretion of higher amount of MELs in the cell suspension incubated with wheat leaf cuttings compared to that in the suspension without cuttings. Transcriptional analysis by real-time reverse transcriptase PCR verified that the expression of erythritol/mannose transferase gene and MELs transporter gene of P. antarctica increased in the cells inoculated onto wheat leaves at 4, 6, and 8 days of incubation, indicating a potential of P. antarctica to produce MELs on the leaves. These findings demonstrate that MELs produced by P. antarctica on plant surfaces could be expected to play a significant role in fungal morphological development and propagation on plant surfaces.

Web of Science ® 被引用回数 : 14

リンク情報
DOI
https://doi.org/10.1007/s00253-014-5675-x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24706213
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000338237400022&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00253-014-5675-x
  • ISSN : 0175-7598
  • eISSN : 1432-0614
  • PubMed ID : 24706213
  • Web of Science ID : WOS:000338237400022

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