論文

査読有り
2014年8月

Production of a biodegradable plastic-degrading enzyme from cheese whey by the phyllosphere yeast Pseudozyma antarctica GB-4(1)W

JOURNAL OF BIOSCIENCE AND BIOENGINEERING
  • Takashi Watanabe
  • ,
  • Yukiko Shinozaki
  • ,
  • Ken Suzuki
  • ,
  • Motoo Koitabashi
  • ,
  • Shigenobu Yoshida
  • ,
  • Yuka Sameshima-Yamashita
  • ,
  • Hiroko Kuze Kitamoto

118
2
開始ページ
183
終了ページ
187
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jbiosc.2014.01.007
出版者・発行元
SOC BIOSCIENCE BIOENGINEERING JAPAN

Cheese whey is a by-product of cheese production and has high concentrations of lactose (about 5%) and other nutrients. Pseudozyma antarctica produces a unique cutinase-like enzyme, named PaE, that efficiently degrades biodegradable plastics. A previous study showed that a combination of 1% oil and 0.5% lactose increased cutinase-like enzyme production by another species of yeast. In this study, to produce PaE from cheese whey, we investigated the effects of soybean oil on PaE production (expressed as biodegradable plastic-degrading activity) by P. antarctica growing on lactose or cheese whey. In flask cultures, the final PaE activity was only 0.03 U/ml when soybean oil was used as the sole carbon source, but increased to 1.79 U/ml when a limited amount of soybean oil (under 0.5%) was combined with a relatively high concentration of lactose (6%). Using a 5-L jar fermentor with lactose fed-batch cultivation and periodic soybean oil addition, about 14.6 U/ml of PaE was obtained after 5 days of cultivation. When the lactose was replaced with cheese whey, PaE production was 10.8 U/ml after 3 days of cultivation. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.

Web of Science ® 被引用回数 : 15

リンク情報
DOI
https://doi.org/10.1016/j.jbiosc.2014.01.007
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24581837
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000341559000012&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.jbiosc.2014.01.007
  • ISSN : 1389-1723
  • eISSN : 1347-4421
  • PubMed ID : 24581837
  • Web of Science ID : WOS:000341559000012

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