論文

査読有り
2014年5月

Purification, characterization, and cloning of the gene for a biodegradable plastic-degrading enzyme from Paraphoma-related fungal strain B47-9

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Ken Suzuki
  • ,
  • Masako Tsujimoto Noguchi
  • ,
  • Yukiko Shinozaki
  • ,
  • Motoo Koitabashi
  • ,
  • Yuka Sameshima-Yamashita
  • ,
  • Shigenobu Yoshida
  • ,
  • Takeshi Fujii
  • ,
  • Hiroko K. Kitamoto

98
10
開始ページ
4457
終了ページ
4465
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00253-013-5454-0
出版者・発行元
SPRINGER

Paraphoma-related fungal strain B47-9 secreted a biodegradable plastic (BP)-degrading enzyme which amounted to 68 % (w/w) of the total secreted proteins in a culture medium containing emulsified poly(butylene succinate-co-adipate) (PBSA) as sole carbon source. The gene for this enzyme was found to be composed of an open reading frame consisting of 681 nucleotides encoding 227 amino acids and two introns. Southern blot analysis showed that this gene exists as a single copy. The deduced amino acid sequence suggested that this enzyme belongs to the cutinase (E.C.3.1.1.74) family; thus, it was named <Emphasis Type="ItalicUnderline">P araphoma-related fungus cutinase-like enzyme (PCLE). It degraded various types of BP films, such as poly(butylene succinate), PBSA, poly(butylene adipate-co-terephthalate), poly(epsilon-caprolactone), and poly(dl-lactic acid). It has a molecular mass of 19.7 kDa, and an optimum pH and temperature for degradation of emulsified PBSA of 7.2 and 45 A degrees C, respectively. Ca2+ ion at a concentration of about 1.0 mM markedly enhanced the degradation of emulsified PBSA.

Web of Science ® 被引用回数 : 27

リンク情報
DOI
https://doi.org/10.1007/s00253-013-5454-0
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24384748
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000335460700016&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00253-013-5454-0
  • ISSN : 0175-7598
  • eISSN : 1432-0614
  • PubMed ID : 24384748
  • Web of Science ID : WOS:000335460700016

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