論文

査読有り
2013年10月

Enzymatic degradation of polyester films by a cutinase-like enzyme from Pseudozyma antarctica: surface plasmon resonance and atomic force microscopy study

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Yukiko Shinozaki
  • ,
  • Yoshihiro Kikkawa
  • ,
  • Shun Sato
  • ,
  • Tokuma Fukuoka
  • ,
  • Takashi Watanabe
  • ,
  • Shigenobu Yoshida
  • ,
  • Toshiaki Nakajima-Kambe
  • ,
  • Hiroko K. Kitamoto

97
19
開始ページ
8591
終了ページ
8598
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00253-012-4673-0
出版者・発行元
SPRINGER

Enzymatic degradation of polyester films by a cutinase-like enzyme from Pseudozyma antarctica JCM10317 (PaE) was analyzed by surface plasmon resonance (SPR). The adsorption of PaE and the degradation rate for polyester films were quantitatively monitored by a positive and negative SPR signal shifts, respectively. The decrease in SPR signal and the erosion depth of amorphous poly(l-lactide) (a-PLLA) film measured by atomic force microscopy (AFM) had a linear relationship, and the weight loss was estimated from the AFM data combined with a density of a-PLLA film. Furthermore, SPR sensorgrams for various polyester films showed that degradation rate of poly(epsilon-caprolactone) and poly(butylene succinate-co-adipate) which contain C6 units was higher than that of other polyesters such as poly(butylene succinate) and a-PLLA. These results suggest that C6 is the preferred chain length as substrates for PaE.

Web of Science ® 被引用回数 : 23

リンク情報
DOI
https://doi.org/10.1007/s00253-012-4673-0
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23339012
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000324552500016&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00253-012-4673-0
  • ISSN : 0175-7598
  • eISSN : 1432-0614
  • PubMed ID : 23339012
  • Web of Science ID : WOS:000324552500016

エクスポート
BibTeX RIS